US 11,832,801 B2
Sweat as a biofluid for analysis and disease identification
Benjamin Katchman, Tempe, AZ (US); Karen Anderson, Scottsdale, AZ (US); and Jennifer Blain Christen, Chandler, AZ (US)
Assigned to ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
Appl. No. 16/317,326
Filed by cARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
PCT Filed Jul. 11, 2017, PCT No. PCT/US2017/041551
§ 371(c)(1), (2) Date Jan. 11, 2019,
PCT Pub. No. WO2018/013579, PCT Pub. Date Jan. 18, 2018.
Claims priority of provisional application 62/360,787, filed on Jul. 11, 2016.
Prior Publication US 2019/0290248 A1, Sep. 26, 2019
Int. Cl. A61B 10/00 (2006.01); G01N 33/569 (2006.01); G01N 21/17 (2006.01)
CPC A61B 10/0064 (2013.01) [G01N 33/56994 (2013.01); G01N 21/17 (2013.01); G01N 2333/05 (2013.01); G01N 2333/11 (2013.01); G01N 2469/20 (2013.01)] 18 Claims
 
1. A method for determining a concentration of a target analyte in sweat, comprising the steps of:
providing between about 1 μL and about 50 μL of a body fluid sample comprising sweat, wherein the sample is collected using a wearable sample collection device worn by a subject while the subject is performing aerobic exercise;
contacting at least a portion of the collected sample to an analyte measurement device comprising an assay unit, wherein the assay unit is configured to detect a plurality of analytes selected from the group consisting of disease-associated IgG antibodies and disease-associated IgA antibodies, and wherein the assay unit comprises reactants for detection of the plurality of analytes;
allowing the collected body fluid sample to react with the reactants, whereby a detectable reaction product is produced if the target analyte is present in the sample; and
detecting and measuring the reaction product, wherein the presence of detectable reaction product indicates the presence of the target analyte in the sample; and wherein the amount of detectable reaction product is related to the concentration of the target analyte in the sample.