US 11,832,633 B2
Protein isolate and process for the production thereof
Claire Defaix, Villers-lés-Nancy (FR); Romain Kapel, Saint Max (FR); and Olivier Galet, Trégueux (FR)
Assigned to AVRIL, Paris (FR); UNIVERSITE DE LORRAINE, Nancy (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR)
Appl. No. 16/764,739
Filed by AVRIL, Paris (FR); UNIVERSITE DE LORRAINE, Nancy (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR)
PCT Filed Nov. 14, 2018, PCT No. PCT/EP2018/081263
§ 371(c)(1), (2) Date May 15, 2020,
PCT Pub. No. WO2019/096862, PCT Pub. Date May 23, 2019.
Claims priority of application No. 17201967 (EP), filed on Nov. 15, 2017; and application No. 17205565 (EP), filed on Dec. 5, 2017.
Prior Publication US 2020/0397018 A1, Dec. 24, 2020
Int. Cl. A23J 1/14 (2006.01); C07K 1/34 (2006.01); C07K 14/76 (2006.01)
CPC A23J 1/142 (2013.01) [A23J 1/148 (2013.01); C07K 1/34 (2013.01); C07K 14/76 (2013.01); C12Y 301/03 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A process for producing a protein isolate from an oilseed meal, said isolate comprising proteins and an amount of 4 wt. % or less of phytic acid, said amount of phytic acid being by weight of proteins in said isolate, said process comprising the following steps:
a) providing cold-pressed, partially defatted, oilseed meal said oilseed meal being selected from the group consisting of rapeseed, canola, flax, lupine, sunflower, safflower, cotton, mustard and hemp seed meals, and mixtures thereof and said oilseed meal comprising from about 5 wt % to about 20 wt % of oil, by total weight of dry matter of partially defatted meal;
b) mixing the oilseed meal with a first aqueous solvent to form a slurry at a pH ranging from 6 to 7.8, said slurry having a solid phase;
c) separating said solid phase from said slurry to obtain a separated solid phase;
d) mixing said separated solid phase with a second aqueous solvent at a pH ranging from 1 to 3.5, to form a mixture said mixture having a liquid phase;
e) separating said liquid phase from said mixture formed in the step d) to obtain a separated liquid phase;
f) f1) mixing the separated liquid phase with a phytase at a temperature and a pH suitable for phytase activity to obtain a mixture having a liquid phase and a solid phase;
and/or
f2) mixing the separated liquid phase with a salt, to obtain a resulting liquid composition having a molar concentration of said salt ranging from 0.05 M to 0.5 M, at a temperature ranging from 40° C. to 70° C., to obtain a mixture having a liquid phase and a solid phase;
g) precipitating a solid phase from the liquid phase of the step f) to obtain a solid precipitate;
h) separating said solid precipitate from the liquid phase of the step g) said liquid phase comprising a water-rich liquid phase and an oil-rich liquid phase;
i) separating said water-rich liquid phase from said oil-rich liquid phase,
j) subjecting said water-rich liquid phase obtained in the step i) to one or several membrane filtration(s) to obtain a protein isolate; and
k) optionally, drying said protein isolate to obtain a dry protein isolate.