	US 12,486,546 B2
	Compositions and methods for amplifying, detecting or quantifying human polyomavirus BK virus
Ankur Shah, San Diego, CA (US); and Meghna Yadav, Carlsbad, CA (US)
Assigned to Gen-Probe Incorporated, San Diego, CA (US)
Filed by Gen-Probe Incorporated, San Diego, CA (US)
Filed on Jun. 21, 2024, as Appl. No. 18/749,697.
Application 18/749,697 is a division of application No. 17/287,178, granted, now 12,054,793, previously published as PCT/US2019/057383, filed on Oct. 22, 2019.
Claims priority of provisional application 62/748,998, filed on Oct. 22, 2018.
Prior Publication US 2024/0336985 A1, Oct. 10, 2024
Int. Cl. C12Q 1/6841 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6853 (2018.01); C12Q 1/70 (2006.01)

CPC C12Q 1/701 (2013.01) [C12Q 1/6841 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6853 (2013.01)]

21 Claims

1. A combination of oligonucleotides for amplifying a BK polyomavirus (BKV) nucleic acid sequence comprising:

a) one or more first amplification primers wherein each first amplification primer is 18-30 nucleobases in length and comprises a nucleotide sequence having at least 90% identity to the nucleotide sequence selected from the group consisting of: SEQ ID NO: 46, and SEQ ID NO:47, and

b) one or more second amplification primers wherein each second amplification primer is 19-30 nucleobases in length and comprises a nucleotide sequence having at least 90% identity to the nucleotide sequence of: SEQ ID NO: 22.