	US 12,486,535 B2
	Ectopic pregnancy kits and methods
Nicole E. Ortogero, Kent, WA (US); Richard O. Burney, University Place, WA (US); Gregory E. Chow, University Place, WA (US); Jessica Lentscher, Lakewood, WA (US); and Zachary Colburn, Lakewood, WA (US)
Assigned to THE GOVERNMENT OF THE UNITED STATES, AS REPRESENTED BY THE SECRETARY OF THE ARMY, Frederick, MD (US)
Filed by The Government of the United States, as Represented by the Secretary of the Army, Fort Detrick, MD (US)
Filed on Mar. 5, 2019, as Appl. No. 16/293,415.
Claims priority of provisional application 62/638,415, filed on Mar. 5, 2018.
Prior Publication US 2019/0390272 A1, Dec. 26, 2019
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6876 (2018.01)

CPC C12Q 1/6876 (2013.01) [C12Q 2600/112 (2013.01); C12Q 2600/158 (2013.01)]

22 Claims

1. A method of treating an ectopic pregnancy in a patient comprising: obtaining results of an applied k-Nearest Neighbor (KNN) algorithm on mRNA expression levels of a set of biomarkers in a biological sample obtained from the patient,

wherein the applied KNN algorithm was trained on expression data obtained from historical patient samples having ectopic or intrauterine pregnancies for the set of biomarkers consisting of: ARMC3, C20orf85, CFAP47, CFAP126, DNAH12, LRRC46, LPAR3, RSPH4A, STOML3, TPPP3, WDR49, and ZBBX, and optionally further consisting of at least one of: AJ239322.3, CHST6, CLEC4M, CNR1, COL3A1, DSP, HLA-DPA1, HPSE2, LINC00890, MS4A8, OMD, OMG, PTGERN, PTGIS, FRFTN2, RNU4-21P, RP11-314M24.1, SNORA72, SORBS1, SPARCL1, WDFY4, and Y RNA.58-201; and

wherein the results indicate that the patient has an ectopic pregnancy and not a normal or abnormal intrauterine pregnancy; and

treating the patient with methotrexate or by surgery.