US 12,486,528 B2
Compositions and methods for measuring and inhibiting calpain-5 activity
Vinit Mahajan, Redwood City, CA (US); Gabriel Velez, Palo Alto, CA (US); Young Joo Sun, Redwood City, CA (US); Kellie A Schaefer, Redwood City, CA (US); and Alexander G Bassuk, Iowa City, IA (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US); University of Iowa Research Foundation, Iowa City, IA (US); and The United States Government as represented by the Department of Veterans Affairs, Washington, DC (US)
Appl. No. 17/767,280
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US); and The United States Government as represented by the Department of Veterans Affairs, Washington, DC (US)
PCT Filed Oct. 9, 2020, PCT No. PCT/US2020/054989
§ 371(c)(1), (2) Date Apr. 7, 2022,
PCT Pub. No. WO2021/072196, PCT Pub. Date Apr. 15, 2021.
Claims priority of provisional application 62/914,253, filed on Oct. 11, 2019.
Prior Publication US 2022/0364142 A1, Nov. 17, 2022
Int. Cl. C12Q 1/37 (2006.01); A61K 45/06 (2006.01); A61K 49/00 (2006.01)
CPC C12Q 1/37 (2013.01) [A61K 45/06 (2013.01); A61K 49/0008 (2013.01); C12Y 304/22 (2013.01); G01N 2333/96466 (2013.01); G01N 2500/02 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A calpain-5 inhibitor selected from the group consisting of:
a) a calpain-5 inhibitor comprising the amino acid sequence of SEQ ID NO:288 or SEQ ID NO:289, wherein the calpain-5 inhibitor comprises a lysine having a methylated epsilon amino group at position 7 of SEQ ID NO:288, or the calpain-5 inhibitor comprises a lysine having a methylated epsilon amino group at position 7 and position 8 of SEQ ID NO:289; and
b) a calpain-5 inhibitor comprising an amino acid sequence having at least 90% identity to the sequence of SEQ ID NO:288 or SEQ ID NO:289, wherein the calpain-5 inhibitor comprises a lysine having a methylated epsilon amino group at position 7 of SEQ ID NO:288, or the calpain-5 inhibitor comprises a lysine having a methylated epsilon amino group at position 7 and position 8 of SEQ ID NO:289.