	US 12,486,526 B2
	Surrogate virus assays and methods
Declan Cosmo Schroeder, Minneapolis, MN (US)
Assigned to REGENTS OF THE UNIVERSITY OF MINNESOTA, Minneapolis, MN (US)
Appl. No. 17/619,220
Filed by REGENTS OF THE UNIVERSITY OF MINNESOTA, Minneapolis, MN (US)
PCT Filed Jun. 23, 2020, PCT No. PCT/US2020/039072 § 371(c)(1), (2) Date Dec. 14, 2021, PCT Pub. No. WO2020/263788, PCT Pub. Date Dec. 30, 2020.
Claims priority of provisional application 62/865,404, filed on Jun. 24, 2019.
Prior Publication US 2022/0235393 A1, Jul. 28, 2022
Int. Cl. C12Q 1/22 (2006.01); A23K 30/00 (2016.01); A23K 50/30 (2016.01); C12Q 1/70 (2006.01)

CPC C12Q 1/22 (2013.01) [A23K 30/00 (2016.05); A23K 50/30 (2016.05); C12Q 1/70 (2013.01)]

14 Claims

1. A method for monitoring the presence or absence of African swine fever virus (ASFV) in animal feed or an animal feed ingredient, the method comprising:

inoculating the animal feed or animal feed ingredient with an ASFV surrogate virus, Emiliania huxleyi virus (EhV), as a proxy for the ASFV;

subjecting the animal feed or animal feed ingredient to a treatment that inactivates the ASFV and the EhV;

waiting a predetermined period of time; and

determining the presence or absence of the EhV in the animal feed or animal feed ingredient, thereby monitoring the presence or absence of the ASFV in the animal feed or animal feed ingredient.