US 12,486,525 B2
Methods for increasing productivity of therapeutic proteins
Jianlin Xu, Devens, MA (US); Shun Zheng, Waltham, MA (US); and Zeinab H Dawood, Devens, MA (US)
Assigned to BRISTOL-MYERS SQUIBB COMPANY, Princeton, NJ (US)
Appl. No. 18/245,753
Filed by BRISTOL-MYERS SQUIBB COMPANY, Princeton, NJ (US)
PCT Filed Sep. 21, 2021, PCT No. PCT/US2021/051185
§ 371(c)(1), (2) Date Mar. 17, 2023,
PCT Pub. No. WO2022/066595, PCT Pub. Date Mar. 31, 2022.
Claims priority of provisional application 63/225,832, filed on Jul. 26, 2021.
Claims priority of provisional application 63/081,380, filed on Sep. 22, 2020.
Prior Publication US 2023/0357815 A1, Nov. 9, 2023
Int. Cl. C12P 21/06 (2006.01); C07K 16/00 (2006.01)
CPC C12P 21/06 (2013.01) [C07K 16/00 (2013.01); C07K 2317/14 (2013.01)] 16 Claims
 
1. A method of producing a therapeutic protein of interest comprising a Fc domain, wherein the Fc domain contains a lysine residue at the C-terminus, the method comprising:
a) culturing host cells expressing the protein under optimized culture conditions in a bioreactor for at least 6 days;
b) adding a carboxypeptidase (CP) to the cultured cells;
c) collecting clarified bulk; and
d) subjecting the clarified bulk to a purification process.