	US 12,486,520 B2
	Insect extracellular vesicles for delivery of nucleic acids
Kristopher Silver, Manhattan, KS (US); and Yoonseong Park, Manhattan, KS (US)
Assigned to Kansas State University Research Foundation, Manhattan, KS (US)
Appl. No. 17/635,036
Filed by Kansas State University Research Foundation, Manhattan, KS (US)
PCT Filed Aug. 14, 2020, PCT No. PCT/US2020/046402 § 371(c)(1), (2) Date Feb. 14, 2022, PCT Pub. No. WO2021/030699, PCT Pub. Date Feb. 18, 2021.
Claims priority of provisional application 62/887,067, filed on Aug. 15, 2019.
Prior Publication US 2022/0356492 A1, Nov. 10, 2022
Int. Cl. C12N 15/88 (2006.01); A01N 63/60 (2020.01); C12N 15/113 (2010.01)

CPC C12N 15/88 (2013.01) [A01N 63/60 (2020.01); C12N 15/113 (2013.01); C12N 2310/14 (2013.01); C12N 2320/32 (2013.01)]

21 Claims

1. A method generating custom extracellular vesicles as delivery vehicles for nucleic acids, said method comprising:

incubating nucleic acid for a target gene with beetle cells in serum-free culture media for an effective period of time; and

collecting extracellular vesicles secreted by said beetle cells, wherein said extracellular vesicles comprise at least a functional fragment of said nucleic acid.