US 12,486,516 B2
Modification of glutamine synthetase to improve yield in plants
Steven Henry Bass, Hillsborough, CA (US); Ericka Bermudez, Aptos, CA (US); Zhenglin Hou, Ankeny, IA (US); Shai Lawit, Urbandale, IA (US); Marian Beecher Sandoval, Oakland, CA (US); Bo Shen, Johnston, IA (US); Peter Thelen, Des Moines, IA (US); Yang Wang, Johnston, IA (US); Robert W Williams, Minneapolis, MN (US); Jingrui Wu, Johnston, IA (US); and Jun Zhang, Johnston, IA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Appl. No. 17/056,817
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
PCT Filed May 20, 2019, PCT No. PCT/US2019/033130
§ 371(c)(1), (2) Date Nov. 19, 2020,
PCT Pub. No. WO2019/226553, PCT Pub. Date Nov. 28, 2019.
Claims priority of provisional application 62/674,250, filed on May 21, 2018.
Prior Publication US 2021/0180079 A1, Jun. 17, 2021
Int. Cl. C12N 15/82 (2006.01); C12N 9/00 (2006.01)
CPC C12N 15/8251 (2013.01) [C12N 9/93 (2013.01); C12N 15/8261 (2013.01); C12Y 603/01002 (2013.01)] 16 Claims
 
1. A polynucleotide encoding a glutamine synthetase (GS) polypeptide comprising an amino acid sequence that is at least 98% identical to SEQ ID NO: 135 wherein the amino acid sequence does not comprise at least one of a leucine corresponding to position 3 of SEQ ID NO: 135, a serine corresponding to position 5 of SEQ ID NO: 135, an isoleucine corresponding to position 8 of SEQ ID NO: 135, an asparagine corresponding to position 11 of SEQ ID NO: 135, an aspartic acid corresponding to position 14 of SEQ ID NO: 135, a threonine corresponding to position 15 of SEQ ID NO: 135, a lysine corresponding to position 18 of SEQ ID NO: 135, an isoleucine corresponding to position 26 of SEQ ID NO: 135, a serine corresponding to position 29 of SEQ ID NO: 135, a leucine corresponding to position 33 of SEQ ID NO: 135, a proline corresponding to position 41 of SEQ ID NO: 135, a tyrosine corresponding to position 55 of SEQ ID NO: 135, an arginine corresponding to position 79 of SEQ ID NO: 135, an arginine corresponding to position 84 of SEQ ID NO: 135, a histidine corresponding to position 108 of SEQ ID NO: 135, a valine corresponding to position 113 of SEQ ID NO: 135, an aspartic acid corresponding to position 118 of SEQ ID NO: 135, a tyrosine corresponding to position 126 of SEQ ID NO: 135, a valine corresponding to position 161 of SEQ ID NO: 135, an isoleucine corresponding to position 171 of SEQ ID NO: 135, a valine corresponding to position 172 of SEQ ID NO: 135, a valine corresponding to position 230 of SEQ ID NO: 135, a lysine corresponding to position 278 of SEQ ID NO: 135, a glutamic acid corresponding to position 286 of SEQ ID NO: 135, a leucine corresponding to position 305 of SEQ ID NO: 135, or an aspartic acid corresponding to position 320 of SEQ ID NO: 135 and the encoded GS polypeptide has increased activity as compared to the polypeptide of SEQ ID NO: 2.