	US 12,486,500 B2
	Method for stimulating the gene expression in endometrial cells
Gianantonio Pozzato, Vicenza (IT); and Alessandro Pozzato, Sandrigo (IT)
Assigned to Telea Medical Group S.R.L., Sandrigo (IT)
Filed by Telea Medical Group S.R.L., Sandrigo (IT)
Filed on Aug. 4, 2020, as Appl. No. 16/984,969.
Claims priority of application No. 102019000013992 (IT), filed on Aug. 5, 2019.
Prior Publication US 2021/0047631 A1, Feb. 18, 2021
Int. Cl. C12N 13/00 (2006.01); A61B 17/425 (2006.01); A61N 1/36 (2006.01); C12N 5/071 (2010.01); C12N 15/67 (2006.01); C07K 14/54 (2006.01); C07K 14/705 (2006.01)

CPC C12N 13/00 (2013.01) [A61B 17/425 (2013.01); A61N 1/36 (2013.01); A61N 1/36034 (2017.08); C12N 5/0682 (2013.01); C12N 15/67 (2013.01); C07K 14/54 (2013.01); C07K 14/705 (2013.01)]

5 Claims

1. An in vitro process for increasing expression of a gene in endometrial cells, comprising the steps of:

contacting a culture of endometrial cells with electrodes and applying electric current waves having a sinusoidal waveform having a fundamental frequency higher than 2 MHz to about 4 MHz for a predetermined amount of time; and

assaying the contacted cells for the expression of a gene, wherein the gene is selected from the group consisting of HOXA10, LIF, ITGB3, HOXA11, and ITGAV, and wherein expression of the gene is increased in response to the electric current waves applied to the endometrial cells.