	US 12,486,483 B2
	Modified treatment chamber for treating cells
Alexander Mathys, Zurich (CH); Leandro Buchmann, Winterthur (CH); Erika Sylvie Georget, Zurich (CH); and Jana Carmen Bünder, Baldegg (CH)
Assigned to Bühler AG, Uzwil (SE)
Appl. No. 17/797,866
Filed by Bühler AG, Uzwil (CH)
PCT Filed Feb. 4, 2021, PCT No. PCT/EP2021/052674 § 371(c)(1), (2) Date Aug. 5, 2022, PCT Pub. No. WO2021/156373, PCT Pub. Date Aug. 12, 2021.
Claims priority of application No. 20156009 (EP), filed on Feb. 7, 2020.
Prior Publication US 2023/0067450 A1, Mar. 2, 2023
Int. Cl. C12M 1/42 (2006.01); C12M 1/00 (2006.01); C12N 13/00 (2006.01)

CPC C12M 35/02 (2013.01) [C12M 47/06 (2013.01); C12N 13/00 (2013.01)]

16 Claims

1. A treatment unit having an inlet and an outlet, and a treatment space being formed inside the treatment unit such that it can be penetrated by electric pulses, said treatment space being fluidly connected with the inlet and the outlet,

wherein

at least a portion of an inner side face of the treatment space is non-planar, and

wherein the inlet is formed in a top face of the treatment unit, and the outlet is formed in a bottom face of the treatment unit, and

wherein the flow of a fluid protruding through said treatment space is homogenized in a zone where the electric field is applied.