US 12,486,304 B2
Peptide ligand targeting carbonic anhydrase IX, peptide construct comprising same, and uses thereof
Song Gil Lee, Gyeongsangbuk-do (KR); Hye Sook Ha, Gyeongsangbuk-do (KR); Jin Hwi Park, Gyeongsangbuk-do (KR); Se Won Kim, Seoul (KR); Yoo Joung Oh, Gyeongsangbuk-do (KR); Seo Hyun Park, Gyeongsangbuk-do (KR); Shin Young Park, Gyeongsangbuk-do (KR); and Jun Hoe Cha, Seoul (KR)
Assigned to C-BIOMEX CO., LTD., Gyeongsangbuk-do (KR)
Appl. No. 18/289,027
Filed by C-BIOMEX CO., LTD., Gyeongsangbuk-do (KR)
PCT Filed Feb. 10, 2023, PCT No. PCT/KR2023/001951
§ 371(c)(1), (2) Date Oct. 31, 2023,
PCT Pub. No. WO2023/153847, PCT Pub. Date Aug. 17, 2023.
Claims priority of application No. 10-2022-0018467 (KR), filed on Feb. 11, 2022.
Prior Publication US 2024/0327464 A1, Oct. 3, 2024
Int. Cl. C07K 7/64 (2006.01); A61K 47/64 (2017.01); A61K 49/00 (2006.01); A61K 51/08 (2006.01); A61P 35/00 (2006.01); C07K 1/107 (2006.01)
CPC C07K 7/64 (2013.01) [A61K 47/64 (2017.08); A61K 49/0056 (2013.01); A61K 51/088 (2013.01); A61P 35/00 (2018.01); C07K 1/1077 (2013.01)] 18 Claims
 
1. A CAIX-specific peptide construct comprising a peptide ligand consisting of any one of amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 44, wherein at least one of the constituent amino acids is composed of D-amino acids and a lysine (Lys) residue among the constituent amino acids may be substituted with a chemical functional group at the side chain ε-amino group; and
a sulfonamide functional group-containing amino acid residue linked to the peptide ligand directly or through a spacer;
wherein the peptide construct has a cyclic structure represented by Formula 1 below:

OG Complex Work Unit Chemistry
wherein,
P is the peptide ligand,
F1 is a glycine (Gly) or a sulfonamide functional group-containing amino acid residue,
F2 is a sulfonamide functional group-containing amino acid residue,
F3 is a glycine (Gly) or a functional group-containing amino acid residue other than sulfonamide,
n and m are each independently 0 or 1,
F4 is a group of the general formula —(S1)o—(F5)p—(S2)q—(F6)r—NH2, wherein
S1 and S2 are each independently a spacer,
F5 and F6 are each independently a functional group-containing amino acid residue other than sulfonamide,
wherein the functional group other than sulfonamide is chelator, cycloalkane having 5 to 15 carbon atoms, biotin, glucoheptonic acid, 4-(p-iodophenyl) butyric acid (IB), fluorescent dye, or cytotoxic agent, and
o, p, q and r each independently represent an integer of 0 to 6.