| CPC A61M 5/3286 (2013.01) [A61M 25/02 (2013.01); A61M 2025/028 (2013.01); A61M 2202/0007 (2013.01); A61M 2210/0612 (2013.01)] | 19 Claims |

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1. A device for injection into an interlayer of an organ of a subject, the device comprising:
a needle comprising a needle lumen extending between a needle proximal end and a needle distal end, said needle distal end including a size transitional portion terminating in a sharp needle distal tip;
an elongated tissue separator having a separator distal tip, at least a portion of said elongated tissue separator being shiftable in the needle lumen; and
an actuator configured to shift said elongated tissue separator in said needle lumen between a first position, wherein said separator distal tip is fixedly positioned proximally to said needle distal tip, and a second position, wherein said separator distal tip is fixedly positioned a first predetermined distance distally from said needle distal tip; and
a cannula fluidly connectable between a syringe and said needle lumen, so that a fluid injected from said syringe is directly flowable to said needle distal tip via said cannula and said needle lumen.
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