	US 12,485,188 B2
	Recombinant adeno-associated virus with modified AAV capsid polypeptides
Yanqun Shu, Shanghai (CN); Jinzhao Hou, Shanghai (CN); Heping Li, Shanghai (CN); Qiongqiong Ren, Shanghai (CN); Lumei Xiao, Shanghai (CN); Bing Wu, Shanghai (CN); and Peilu Li, Shanghai (CN)
Assigned to Skyline Therapeutics Limited, Cayman Islands (GB)
Filed by Skyline THerapeutics Limited, Cayman Islands (GB)
Filed on Aug. 16, 2024, as Appl. No. 18/807,211.
Application 18/807,211 is a continuation of application No. PCT/CN2023/076389, filed on Feb. 16, 2023.
Claims priority of application No. PCT/CN2022/076619 (WO), filed on Feb. 17, 2022.
Prior Publication US 2024/0398992 A1, Dec. 5, 2024
Int. Cl. A61K 48/00 (2006.01); C07K 14/005 (2006.01); C07K 16/22 (2006.01); C12N 15/86 (2006.01)

CPC A61K 48/0058 (2013.01) [C07K 14/005 (2013.01); C07K 16/22 (2013.01); C12N 15/86 (2013.01); C12N 2750/14122 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14145 (2013.01); C12N 2750/14152 (2013.01); C12N 2750/14171 (2013.01)]

20 Claims

1. A modified adeno-associated virus (AAV) capsid polypeptide comprising, as compared to the parental AAV capsid polypeptide, a peptide inserted into loop IV, and wherein the inserted peptide comprises the amino acid sequence of Formula I:

spacer1-X1-X2-X3-X4-X5-X6-X7-spacer2 (I),

wherein the spacers 1 and 2 independently comprise one or more amino acids selected from A, L and G, and wherein X1-X2-X3-X4-X5-X6-X7 is the amino acid sequence selected from SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8 and SEQ ID NO: 9.