US 12,484,574 B2
Agent for promoting undifferentiation and cryoprotective agent using aprotic zwitterion
Kosuke Kuroda, Kanazawa (JP); and Eishu Hirata, Kanazawa (JP)
Assigned to NATIONAL UNIVERSITY CORPORATION KANAZAWA UNIVERSITY, Kanazawa (JP)
Appl. No. 17/609,927
Filed by JAPAN SCIENCE AND TECHNOLOGY AGENCY, Kawaguchi (JP)
PCT Filed May 8, 2020, PCT No. PCT/JP2020/018668
§ 371(c)(1), (2) Date May 27, 2022,
PCT Pub. No. WO2020/230721, PCT Pub. Date Nov. 19, 2020.
Claims priority of application No. 2019-090509 (JP), filed on May 13, 2019.
Prior Publication US 2022/0287294 A1, Sep. 15, 2022
Int. Cl. A01N 1/125 (2025.01); C09K 3/18 (2006.01); C12N 5/00 (2006.01)
CPC A01N 1/125 (2025.01) [C09K 3/18 (2013.01); C12N 5/525 (2025.01)] 5 Claims
 
1. A medium for cryopreservation, comprising an aqueous solution of an aprotic zwitterion represented by formula (4):
R4-X—R2-A  (4)
wherein R4 is an alkyl group having 1 to 7 carbon atoms, an alkenyl group having 1 to 7 carbon atoms, or an alkyl group having 1 to 7 carbon atoms and containing an ether bond, X is a cation moiety of the zwitterion and represents a cation selected from the group consisting of an imidazolium cation, a phosphonium cation, a sulfonium cation, a pyrazolium cation, a pyridinium cation, a pyrrolidinium cation, a morpholinium cation, a cyclopropenylium cation and a piperidinium cation, A represents an anion moiety and represents an anion selected from the group consisting of SO3−, —COO−, —OP═O(H)O−, —OP═O(CH3)O− and —OP═O(OR3)O−, R2 is an alkylene group having 1 to 5 carbon atoms and optionally having a substituent, and R3 is hydrogen or an alkyl group optionally having a heteroatom in the molecular chain, and a cell-permeable substance, wherein the amount of the cell-permeable substance is from 1 to 30 parts by weight per 100 parts by weight of the aqueous solution of the aprotic zwitterion.