US 12,153,010 B2
Multiwell electrode-based biosensor
Kyuhong Lee, Jeollabuk-do (KR)
Assigned to KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY, Deajeon (KR)
Appl. No. 17/048,002
Filed by Korea Research Institute of Chemical Technology, Daejeon (KR)
PCT Filed Apr. 10, 2019, PCT No. PCT/KR2019/004259
§ 371(c)(1), (2) Date Oct. 15, 2020,
PCT Pub. No. WO2019/203493, PCT Pub. Date Oct. 24, 2019.
Claims priority of application No. 10-2018-0044218 (KR), filed on Apr. 17, 2018.
Prior Publication US 2021/0140912 A1, May 13, 2021
Int. Cl. G01N 27/327 (2006.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01)
CPC G01N 27/3277 (2013.01) [G01N 27/3276 (2013.01); G01N 27/3278 (2013.01); G01N 33/5438 (2013.01); G01N 33/68 (2013.01)] 6 Claims
OG exemplary drawing
 
1. An electrochemical amperometric biosensor comprising:
an electrode in which one or more nano-wells for accommodating a sample are formed, wherein a diameter of the one or more nano-wells is from 300 nm to 400 nm;
a first probe provided on the electrode and an adaptor protein that specifically binds to the first probe, wherein the adaptor protein is immobilized on the electrode exposed at a bottom portion of the nano-well;
a second probe that specifically binds to a target substance that binds to the first probe; and
a signal mediator molecule that specifically binds to the second probe,
wherein the second probe is bound to a protein tag;
wherein the electrode before the first probe is provided is surface-modified into a self-assembled monolayer (SAM);
wherein the first probe specifically binds to the adaptor protein immobilized on the electrode exposed at the bottom portion of the nano-well;
wherein the signal mediator comprises a protein that specifically binds to the protein tag and a peroxidase; and
wherein the biosensor is configured to measure a concentration of the target substance in the sample by an amperometry method.