US 12,152,991 B2
Time-resolved laser-induced fluorescence spectroscopy systems and uses thereof
Pramod Butte, Studio City, CA (US); Paul Lapchak, San Diego, CA (US); and David Scott Kittle, Running Springs, CA (US)
Assigned to Cedars-Sinai Medical Center, Los Angeles, CA (US)
Filed by Cedars-Sinai Medical Center, Los Angeles, CA (US)
Filed on Jul. 21, 2022, as Appl. No. 17/870,090.
Application 17/870,090 is a continuation of application No. 17/198,816, filed on Mar. 11, 2021, granted, now 11,428,636.
Application 17/198,816 is a continuation of application No. 16/382,791, filed on Apr. 12, 2019, granted, now 10,983,060, issued on Apr. 20, 2021.
Application 16/382,791 is a continuation of application No. 15/196,354, filed on Jun. 29, 2016, granted, now 10,288,567, issued on May 14, 2019.
Application 15/196,354 is a continuation of application No. 14/776,086, granted, now 9,404,870, issued on Aug. 2, 2016, previously published as PCT/US2014/030610, filed on Mar. 17, 2014.
Claims priority of provisional application 61/794,741, filed on Mar. 15, 2013.
Prior Publication US 2023/0228683 A1, Jul. 20, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 21/64 (2006.01); A61B 5/00 (2006.01); G01J 3/02 (2006.01); G01J 3/28 (2006.01); G01J 3/44 (2006.01); A61B 5/1455 (2006.01); A61B 5/1459 (2006.01)
CPC G01N 21/6486 (2013.01) [A61B 5/0071 (2013.01); G01J 3/0218 (2013.01); G01J 3/2889 (2013.01); G01J 3/4406 (2013.01); G01N 21/64 (2013.01); G01N 21/6402 (2013.01); G01N 21/6408 (2013.01); G01N 21/6428 (2013.01); G01N 21/645 (2013.01); A61B 5/0084 (2013.01); A61B 5/14556 (2013.01); A61B 5/1459 (2013.01); A61B 5/4866 (2013.01); G01N 2021/6417 (2013.01); G01N 2021/6421 (2013.01); G01N 2021/6484 (2013.01); G01N 2201/06113 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A method for characterizing a biological sample by analyzing emission of a fluorescence signal from a biological sample upon excitation, comprising:
(a) irradiating the biological sample with a light source at a predetermined wavelength to cause the biological sample to produce a responsive fluorescence signal;
(b) collecting the responsive fluorescence signal from the biological sample, wherein the responsive fluorescence signal is split based on a wavelength of one or more wavelength-splitting devices; and
(c) detecting the responsive fluorescence signal with a detector, wherein a gain of the detector is dynamically changed based on the responsive fluorescence signal.