	US 12,152,317 B2
	Transferrin receptor-binding molecules, conjugates thereof and their uses
Romy Cohen, Marseilles (FR); Marion David, Marseilles (FR); and Michel Khrestchatisky, Marseilles (FR)
Assigned to VECT-HORUS, Marseilles (FR); UNIVERSITE D'AIX-MARSEILLE, Marseilles (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR)
Appl. No. 17/421,412
Filed by VECT-HORUS, Marseilles (FR); UNIVERSITE D'AIX-MARSEILLE, Marseilles (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR)
PCT Filed Jan. 8, 2020, PCT No. PCT/EP2020/050318 § 371(c)(1), (2) Date Jul. 8, 2021, PCT Pub. No. WO2020/144233, PCT Pub. Date Jul. 16, 2020.
Claims priority of application No. 19305031 (EP), filed on Jan. 9, 2019.
Prior Publication US 2022/0090050 A1, Mar. 24, 2022
Int. Cl. C40B 40/10 (2006.01); C07K 16/28 (2006.01); C12N 15/10 (2006.01)

CPC C40B 40/10 (2013.01) [C07K 16/2881 (2013.01); C12N 15/1037 (2013.01); C07K 2317/24 (2013.01); C07K 2317/569 (2013.01); C07K 2317/92 (2013.01)]

14 Claims

1. A VHH molecule of formula FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4, wherein said VHH molecule binds both a human and a non-human animal transferrin receptor (TfR) with an affinity (Kd) comprised between 0.1 nM and 10 μM, optionally conjugated to at least one molecule or scaffold, wherein the CDR1 sequence, the CDR2 sequence, and the CDR3 sequence comprise SEQ ID NOs: 1, 2 and 3, respectively; or SEQ ID NOs: 5, 6 and 7, respectively; or SEQ ID NOs: 9, 10 and 11, respectively; or SEQ ID NOs: 13, 14 and 15, respectively; SEQ ID NOs: 17, 2 and 3, respectively; or SEQ ID NOs: 19, 2 and 3, respectively; or SEQ ID NOs: 1, 21 and 3, respectively; or SEQ ID NOs: 1, 23 and 3, respectively; or SEQ ID NOs: 1, 2 and 25, respectively; or SEQ ID NOs: 1, 2 and 27, respectively; or SEQ ID NOs: 1, 2 and 29, respectively; or SEQ ID NOs: 1, 2 and 31, respectively; or SEQ ID NOs: 1, 2 and 33, respectively; or SEQ ID NOs: 67, 2 and 3, respectively; or SEQ ID NOs: 69, 2 and 3, respectively; or SEQ ID NOs: 1, 71 and 3, respectively; or SEQ ID NOs: 1, 73 and 3, respectively; or SEQ ID NOs: 1, 75 and 3, respectively; or SEQ ID NOs: 1, 2 and 77, respectively; or SEQ ID NOs: 1, 2 and 79, respectively; or SEQ ID NOs: 1, 2 and 81, respectively; or SEQ ID NOs: 1, 2 and 83, respectively; or SEQ ID NOs: 1, 2 and 85, respectively.