US 12,152,272 B2
Fluidic cartridge with valve mechanism
David Eberhart, Santa Clara, CA (US); William D. Nielsen, San Jose, CA (US); Helen Franklin, Santa Clara, CA (US); Stevan B. Jovanovich, Livermore, CA (US); Dennis Lehto, Santa Clara, CA (US); Kaiwan Chear, Livermore, CA (US); James Klevenberg, Livermore, CA (US); Chungsoo Charles Park, Fremont, CA (US); Corey M. Smith, Los Altos, CA (US); Philip Justus Wunderle, Oakland, CA (US); and David King, Menlo Park, CA (US)
Assigned to IntegenX Inc., Pleasanton, CA (US)
Filed by IntegenX Inc., Pleasanton, CA (US)
Filed on Dec. 4, 2023, as Appl. No. 18/527,656.
Application 17/181,228 is a division of application No. 16/250,646, filed on Jan. 17, 2019, granted, now 10,961,561, issued on Mar. 30, 2021.
Application 16/250,646 is a division of application No. 15/117,053, granted, now 10,208,332, issued on Feb. 19, 2019, previously published as PCT/US2015/028510, filed on Apr. 30, 2015.
Application 18/527,656 is a continuation of application No. 17/181,228, filed on Feb. 22, 2021, granted, now 11,891,650.
Claims priority of provisional application 62/069,752, filed on Oct. 28, 2014.
Claims priority of provisional application 62/069,473, filed on Oct. 28, 2014.
Claims priority of provisional application 62/067,429, filed on Oct. 22, 2014.
Claims priority of provisional application 62/067,120, filed on Oct. 22, 2014.
Claims priority of provisional application 62/067,404, filed on Oct. 22, 2014.
Claims priority of provisional application 62/001,533, filed on May 21, 2014.
Prior Publication US 2024/0175075 A1, May 30, 2024
Int. Cl. C12Q 1/6806 (2018.01); B01L 3/00 (2006.01); C12Q 1/686 (2018.01); G01N 27/447 (2006.01)
CPC C12Q 1/6806 (2013.01) [B01L 3/502707 (2013.01); B01L 3/502715 (2013.01); B01L 3/50273 (2013.01); B01L 3/502738 (2013.01); C12Q 1/686 (2013.01); G01N 27/44743 (2013.01); G01N 27/44791 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/12 (2013.01); B01L 2300/041 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0848 (2013.01); B01L 2300/087 (2013.01); B01L 2300/123 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0478 (2013.01)] 20 Claims
OG exemplary drawing
 
1. An instrument comprising a cartridge interface and a cartridge engaged with the cartridge interface, wherein:
a cartridge comprising:
a body comprising a malleable material, and
a layer comprising a deformable material bonded to a surface of the body and sealing one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body,
wherein:
the one or more valve bodies comprise:
a segment of the channel comprising a wall having a pair of ridges and a floor depressed into the surface, and
reliefs depressed into the surface and defining depressions that flank the ridges on two sides of the segment of the channel; and
the layer is bonded to the surface of the body and to the ridges such that the one or more channels, channel segments and reliefs are sealed, and
wherein a valve body sealed with the layer forms a valve closable by forcing the layer against the floor of the segment of the channel; and
a cartridge interface comprising a plurality of rams, each ram positioned to actuate a valve of the cartridge and comprising a head having a widest dimension that is wider than a channel segment and less wide than a width between the reliefs, so that, when actuated toward the valve, the head presses the deformable material against the ridges and the floor, thereby closing the valve, but clearing the reliefs.