US 12,152,229 B2
Electroporation apparatus and method
Declan Soden, Clonakilty (IE); Colin Forde, Kinvara (IE); Sean Kinsella, Oranmore (IE); and Tony Moore, Ballincollig (IE)
Assigned to MIRAI MEDICAL LIMITED, Oranmore (IE)
Filed by MIRAI MEDICAL LIMITED, Oranmore (IE)
Filed on Oct. 18, 2023, as Appl. No. 18/381,368.
Application 18/381,368 is a division of application No. 17/636,649, granted, now 11,912,975, previously published as PCT/EP2020/074374, filed on Sep. 1, 2020.
Claims priority of application No. 19194958 (EP), filed on Sep. 2, 2019; application No. 19194959 (EP), filed on Sep. 2, 2019; and application No. 20168567 (EP), filed on Apr. 7, 2020.
Prior Publication US 2024/0076599 A1, Mar. 7, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/42 (2006.01); A61B 18/00 (2006.01); A61N 1/32 (2006.01)
CPC C12M 35/02 (2013.01) [A61N 1/327 (2013.01); A61B 2018/00613 (2013.01); A61B 2018/00875 (2013.01)] 18 Claims
OG exemplary drawing
 
1. An electroporation apparatus comprising:
a plurality of electroporation probe terminals;
a plurality of probe electrodes connected to said probe terminals;
said probe electrodes being adapted for inserting into a biological load;
a transformer for providing stepped-up voltage,
a switching circuit with switches for linking high voltage and low voltage or ground levels to the probe terminals; and
a controller configured to control said switches according to a control scheme to deliver pulses to the probe terminals, and in which said pulses are delivered to groups of at least two probe electrode terminals for said probe electrodes which are spaced apart,
wherein said pulses include bipolar voltage pulses for a pair of electrodes;
wherein the apparatus is configured to:
a) deliver bipolar pulses with a frequency in the range of 100 kHz and 500 kHz with a pulse plateau duration in the range of 1 μs to 5 μs and a voltage amplitude in the range of 100 V/cm to 3000 V/cm, and
b) deliver a monopolar pulse with a voltage in the range of 1 V/cm to 200 V/cm and a pulse duration in the range of 10 ms to 10 s.