| CPC C12M 23/14 (2013.01) [C12M 25/06 (2013.01); C12M 37/04 (2013.01)] | 19 Claims |

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1. A single-use flexible bioreactor bag, comprising:
a rigid multiport plate sealed to an inside of a side wall of said bioreactor bag, wherein said multiport plate comprises an outside surface facing the side wall of said bioreactor bag, an inside surface opposing said outside surface facing an interior volume of said bioreactor bag and a plurality of preformed plate apertures extending between said outside surface and said inside surface, wherein said outside surface comprises a plurality of ports configured to extend outward through said side wall of said bioreactor bag; and
a back film coupled to said inside surface of said multiport plate, said back film having a plurality of preformed back film apertures aligned correspondingly with the plurality of preformed plate apertures in said multiport plate, wherein the plurality of preformed back film apertures are separate and distinct from the plurality of preformed plate apertures.
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