US 12,152,079 B2
Nucleic acids encoding IL-13 receptor alpha 2 (IL13Ra2) chimeric antigen receptor for tumor specific T cell immunotherapy
Giacomo Tampella, Seattle, WA (US); and Michael C. Jensen, Seattle, WA (US)
Assigned to Seattle Children's Research Institute, Seattle, WA (US)
Appl. No. 16/979,471
Filed by Seattle Children's Hospital, Seattle, WA (US)
PCT Filed Mar. 12, 2019, PCT No. PCT/US2019/021823
§ 371(c)(1), (2) Date Sep. 9, 2020,
PCT Pub. No. WO2019/178078, PCT Pub. Date Sep. 19, 2019.
Claims priority of provisional application 62/643,055, filed on Mar. 14, 2018.
Prior Publication US 2021/0000875 A1, Jan. 7, 2021
Int. Cl. C07K 16/28 (2006.01); A61K 35/17 (2015.01); A61K 39/39 (2006.01); A61K 39/395 (2006.01); A61P 35/00 (2006.01); C07K 14/705 (2006.01); C07K 14/725 (2006.01)
CPC C07K 16/2866 (2013.01) [A61K 35/17 (2013.01); A61K 39/3955 (2013.01); A61P 35/00 (2018.01); C07K 14/7051 (2013.01); C07K 14/70521 (2013.01); C07K 14/70578 (2013.01); C07K 2319/03 (2013.01)] 16 Claims
 
1. A nucleic acid encoding a chimeric antigen receptor, the chimeric antigen receptor comprising:
a ligand binding domain that binds to an IL-13 alpha 2 (IL13Rα2) receptor, wherein the ligand binding domain comprises:
a heavy chain variable (VH) domain comprising
a complementarity determining region (CDR) 1 having the amino acid sequence set forth in SEQ ID NO: 20;
a CDR2 having the amino acid sequence set forth in SEQ ID NO: 21; and
a CDR3 having the amino acid sequence set forth in SEQ ID NO: 22; and
a light chain variable (VL) domain comprising
a CDR1 having the amino acid sequence set forth in SEQ ID NO: 23;
a CDR2 having the amino acid sequence set forth in SEQ ID NO: 24; and
a CDR3 having the amino acid sequence set forth in SEQ ID NO: 25;
a polypeptide spacer between the ligand binding domain and a transmembrane domain, the polypeptide spacer having at least 95% identity with the amino acid sequence of SEQ ID NO:09;
the transmembrane domain; and
an intracellular signaling region,
wherein the transmembrane domain links the polypeptide spacer to the intracellular signaling region.