	US 12,152,069 B2
	Nucleic acid molecules and applications thereof in human antibody
Zuohua Liu, Chongqing (CN); Liangpeng Ge, Chongqing (CN); Yuchun Ding, Chongqing (CN); Xiangang Zou, Chongqing (CN); Songquan Yang, Chongqing (CN); Xiaoyan You, Chongqing (CN); Xueqin Liu, Chongqing (CN); and Meng Wu, Chongqing (CN)
Assigned to CHONGQING JINMAIBO BIOTEC CO., LTD, Chongqing (CN)
Appl. No. 17/041,460
Filed by CHONGQING JINMAIBO BIOTEC CO., LTD, Chongqing (CN)
PCT Filed Apr. 16, 2018, PCT No. PCT/CN2018/083163 § 371(c)(1), (2) Date Sep. 25, 2020, PCT Pub. No. WO2019/184015, PCT Pub. Date Oct. 3, 2019.
Claims priority of application No. 201810261091.2 (CN), filed on Mar. 27, 2018.
Prior Publication US 2021/0009670 A1, Jan. 14, 2021
Int. Cl. C07K 16/18 (2006.01); A01K 67/0275 (2024.01); C12N 15/85 (2006.01)

CPC C07K 16/18 (2013.01) [A01K 67/0275 (2013.01); C12N 15/85 (2013.01); A01K 2217/052 (2013.01); A01K 2217/15 (2013.01); A01K 2227/105 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C12N 2015/8518 (2013.01)]

7 Claims

1. Nucleic acid molecules comprising immunoglobulin genes or parts of the immunoglobulin genes, wherein the nucleic acid molecules comprise:

an IgH heavy chain 5′-enhancer,

an IgM gene comprising IgHCμ and an IgM switch region comprising Sμ derived from a transgenic host animal,

wherein the transgenic host animal is a mouse; and

further comprising IgG genes (Igγ), wherein the IgG genes are transgenic host animal/human chimeric sequences comprising human subtypes Igγ3, Igγ1, Igγ2, and Igγ4 and mouse subtypes Igγ3, Igγ1, Igγ2a and Igγ2b.