US 12,152,054 B2
Methods of purifying polypeptides
Hui F. Liu, Oceanside, CA (US); Brian David Kelley, Burlingame, CA (US); Deanna E. Myers, Encinitas, CA (US); Beth McCooey, Oceanside, CA (US); and Krista Marie Petty, San Jose, CA (US)
Assigned to Genentech, Inc., South San Francisco, CA (US)
Filed by Genentech, Inc., South San Francisco, CA (US)
Filed on Jul. 7, 2021, as Appl. No. 17/369,765.
Application 17/369,765 is a continuation of application No. 16/749,888, filed on Jan. 22, 2020, abandoned.
Application 16/749,888 is a continuation of application No. 16/167,364, filed on Oct. 22, 2018, abandoned.
Application 16/167,364 is a continuation of application No. 13/682,620, filed on Nov. 20, 2012, abandoned.
Application 13/682,620 is a continuation of application No. PCT/US2011/037977, filed on May 25, 2011.
Claims priority of provisional application 61/348,143, filed on May 25, 2010.
Prior Publication US 2022/0064209 A1, Mar. 3, 2022
Int. Cl. C07K 1/18 (2006.01); B01D 15/36 (2006.01); B01D 15/38 (2006.01); C07K 1/16 (2006.01); G01N 30/88 (2006.01)
CPC C07K 1/18 (2013.01) [B01D 15/362 (2013.01); B01D 15/3847 (2013.01); C07K 1/165 (2013.01); G01N 2030/8831 (2013.01)] 27 Claims
 
1. A method for purifying an antibody or immunoadhesin from a composition comprising the antibody or immunoadhesin and at least one contaminant, wherein the method comprises either (i) or (ii):
(i) sequential steps of (a) loading the composition onto a cation exchange material at a loading density of greater than about 150 g/L of cation exchange material; and (b) loading a composition recovered from the cation exchange material as an unbound fraction onto a mixed mode material; or
(ii) sequential steps of (a) loading the composition onto a mixed mode material; and (b) loading a composition recovered from mixed mode material as an unbound fraction onto a cation exchange material at a loading density of greater than about 150 g/L of cation exchange material;
wherein the cation exchange material is resin particles.