US 12,480,950 B2
AKT-specific capture agents, compositions, and methods of using and making
James R. Heath, South Pasadena, CA (US); Arundhati Nag, Pasadena, CA (US); Samir Das, Pasadena, CA (US); Kaycie M. Deyle, Sylmar, CA (US); Steven Wesley Millward, Monrovia, CA (US); and Paul Edward Kearney, Seattle, WA (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US); and California Institute of Technology, Pasadena, CA (US)
Filed by California Institute of Technology, Pasadena, CA (US); and Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Feb. 20, 2019, as Appl. No. 16/280,953.
Application 16/280,953 is a continuation of application No. 14/949,236, filed on Nov. 23, 2015, granted, now 10,221,214.
Application 14/949,236 is a continuation of application No. 13/546,575, filed on Jul. 11, 2012, granted, now 9,239,332.
Claims priority of provisional application 61/598,614, filed on Feb. 14, 2012.
Claims priority of provisional application 61/597,628, filed on Feb. 10, 2012.
Claims priority of provisional application 61/506,560, filed on Jul. 11, 2011.
Prior Publication US 2019/0177367 A1, Jun. 13, 2019
Int. Cl. G01N 33/53 (2006.01); A61K 38/08 (2019.01); A61K 49/00 (2006.01); A61K 51/08 (2006.01); C07K 7/02 (2006.01); C07K 14/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/48 (2006.01); C12Q 1/6804 (2018.01); G01N 33/573 (2006.01); G01N 33/574 (2006.01); A61K 38/00 (2006.01)
CPC G01N 33/57449 (2013.01) [A61K 38/08 (2013.01); A61K 49/0043 (2013.01); A61K 49/0056 (2013.01); A61K 51/08 (2013.01); C07K 7/02 (2013.01); C07K 14/001 (2013.01); C12N 15/1058 (2013.01); C12Q 1/485 (2013.01); C12Q 1/6804 (2013.01); G01N 33/573 (2013.01); G01N 33/5748 (2013.01); G01N 33/57496 (2013.01); A61K 38/00 (2013.01); G01N 2333/91205 (2013.01); G01N 2333/91215 (2013.01)] 35 Claims
 
1. A method of identifying an anchor ligand to an epitope of interest, the method comprising:
contacting a polypeptide fragment with a plurality of candidate peptides, wherein the polypeptide fragment comprises the epitope, wherein the epitope is modified or substituted with (a) a group comprising an azido or acetylene group and (b) a label, wherein the candidate peptides all comprise an acetylene group if the epitope has an azido group or an azido group if the epitope has an acetylene group; and
incubating the polypeptide fragment and the candidate peptides to allow formation of a triazole linkage between the polypeptide fragment and one of the candidate peptides, wherein a candidate peptide that forms a triazole linkage with the polypeptide fragment is identified as the anchor ligand.