	US 12,480,946 B2
	Method and kit for rapid measurement of autoantibody activity with respect to TSH receptor
Atsushi Kawasaki, Chiba (JP); Motoki Hoshina, Chiba (JP); Junken Aoki, Miyagi (JP); Kouki Kawakami, Miyagi (JP); and Asuka Inoue, Miyagi (JP)
Assigned to YAMASA CORPORATION, Chiba (JP); and TOHOKU UNIVERSITY, Miyagi (JP)
Appl. No. 17/272,752
Filed by Yamasa Corporation, Chiba (JP); and Tohoku University, Miyagi (JP)
PCT Filed Sep. 2, 2019, PCT No. PCT/JP2019/034400 § 371(c)(1), (2) Date Mar. 2, 2021, PCT Pub. No. WO2020/050208, PCT Pub. Date Mar. 12, 2020.
Claims priority of application No. 2018-165733 (JP), filed on Sep. 5, 2018; and application No. 2019-020595 (JP), filed on Feb. 7, 2019.
Prior Publication US 2021/0318305 A1, Oct. 14, 2021
Int. Cl. G01N 33/68 (2006.01); G01N 33/564 (2006.01)

CPC G01N 33/564 (2013.01) [G01N 2333/726 (2013.01)]

16 Claims

1. A method for measuring autoantibody activity against a thyroid stimulating hormone (TSH) receptor in a mammal cell, comprising the following steps (a) to (c):

(a) incubating a mammal cell expressing both a luciferase containing CAMP binding region and the TSH receptor in the presence of a blood sample collected from a test subject and a substrate capable of visualizing or quantifying the luciferase containing cAMP binding region;

(b) measuring an activation level of the luciferase containing cAMP binding region after the step (a); and

(c) comparing the activation level measured in the step (b) with an activation level in a control to calculate the autoantibody activity.