US 12,480,945 B2
Systems and methods for utilizing combined magnetic nanoparticles and nanobodies
Jaime Heiss, Menlo Park, CA (US)
Filed by Jaime Heiss, Menlo Park, CA (US)
Filed on Dec. 2, 2022, as Appl. No. 18/074,305.
Claims priority of provisional application 63/285,492, filed on Dec. 3, 2021.
Prior Publication US 2023/0176043 A1, Jun. 8, 2023
Int. Cl. G01N 33/543 (2006.01)
CPC G01N 33/54333 (2013.01) [G01N 2446/20 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for determining a conformational change in a structure of a protein, the method comprising:
delivering a magnetic nanoparticle-nanobody (MNP-NB) complex to a sample containing the protein, wherein the MNP-NB complex will bind to the protein in the sample;
applying an external magnetic field with a magnetic field generation system to the sample;
detecting signal from the MNP-NB complex that reflects a response to the external magnetic field, wherein the detecting comprises performing magnetic particle spectroscopy and/or AC susceptibility measurement; and
determining the conformational change in the structure of the protein by detecting the signal in the sample and comparing the detected signal to a baseline signal obtained prior to the conformational change.