US 12,480,943 B2
Temperature-responsive fluorescent particles for detection of biomolecules
Shinji Takeoka, Tokyo (JP); Keitaro Sou, Tokyo (JP); Runkai Hu, Tokyo (JP); Chi-Lik Ken Lee, Singapore (SG); and Li Yan Chan, Singapore (SG)
Assigned to NANOTHETA INC., Tokyo (JP)
Appl. No. 17/610,565
Filed by NANOTHETA INC., Tokyo (JP)
PCT Filed May 29, 2020, PCT No. PCT/JP2020/021358
§ 371(c)(1), (2) Date Nov. 11, 2021,
PCT Pub. No. WO2020/241830, PCT Pub. Date Dec. 3, 2020.
Claims priority of application No. 2019-100299 (JP), filed on May 29, 2019.
Prior Publication US 2022/0221450 A1, Jul. 14, 2022
Int. Cl. G01N 33/543 (2006.01); C09B 57/00 (2006.01); G01N 21/64 (2006.01); G01K 11/00 (2006.01)
CPC G01N 33/5432 (2013.01) [C09B 57/007 (2013.01); G01N 21/64 (2013.01); G01K 11/00 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A temperature-responsive fluorescent particle comprising a fluorescent molecule in a molecular assembly comprising and constituted by an amphiphilic lipid molecule, wherein the fluorescent molecule is of general formula I:

OG Complex Work Unit Chemistry
wherein R1 represents a hydrophobic group; R2 represents a hydrophobic group; and R1 and R2 may be the same or different,
wherein the fluorescent molecule is quenched when the molecular assembly is in a solid phase and the fluorescent molecule emits fluorescence when the molecular assembly is in a liquid phase, due to a temperature-responsive solid-liquid phase transition,
so that quenching and fluorescence emission of the fluorescent molecule are reversibly switched in a temperature responsive manner.