US 12,480,938 B2
Methods and systems for analyte detection and analysis
Kristopher Barbee, Pleasanton, CA (US); Nathan Beckett, Oakland, CA (US); Denis Pristinski, Dublin, CA (US); Derek Schulte, Los Angeles, CA (US); Avishai Bartov, Hod-Hasharon (IL); Jamie Sullivan, Eugene, OR (US); Dumitru Brinza, Montara, CA (US); Abizar Lakdawalla, Los Altos Hills, CA (US); Steven Menchen, Fremont, CA (US); Gilad Almogy, Palo Alto, CA (US); and Mark Pratt, Bozeman, MT (US)
Assigned to Ultima Genomics, Inc., Fremont, CA (US)
Filed by Ultima Genomics, Inc., Fremont, CA (US)
Filed on Jul. 17, 2024, as Appl. No. 18/776,046.
Application 18/776,046 is a continuation of application No. 18/224,378, filed on Jul. 20, 2023, granted, now 12,188,924.
Application 18/224,378 is a continuation of application No. 17/962,163, filed on Oct. 7, 2022, granted, now 11,747,323, issued on Sep. 5, 2023.
Application 17/962,163 is a continuation of application No. 16/862,196, filed on Apr. 29, 2020, granted, now 11,499,962, issued on Nov. 15, 2022.
Application 16/862,196 is a continuation of application No. PCT/US2018/061598, filed on Nov. 16, 2018.
Application PCT/US2018/061598 is a continuation in part of application No. 15/974,364, filed on May 8, 2018, granted, now 10,344,328, issued on Jul. 9, 2019.
Application PCT/US2018/061598 is a continuation in part of application No. 15/974,543, filed on May 8, 2018, granted, now 10,273,528, issued on Apr. 30, 2019.
Application PCT/US2018/061598 is a continuation in part of application No. 15/974,441, filed on May 8, 2018, granted, now 10,267,790, issued on Apr. 23, 2019.
Claims priority of provisional application 62/664,049, filed on Apr. 27, 2018.
Claims priority of provisional application 62/623,743, filed on Jan. 30, 2018.
Claims priority of provisional application 62/588,139, filed on Nov. 17, 2017.
Prior Publication US 2025/0012778 A1, Jan. 9, 2025
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/49 (2006.01); G01N 1/02 (2006.01); G01Q 10/04 (2010.01); G01N 1/22 (2006.01)
CPC G01N 33/492 (2013.01) [G01N 1/02 (2013.01); G01Q 10/04 (2013.01); G01N 2001/2291 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for processing a plurality of biological analytes, comprising:
(a) rotating a substrate affixed to a chuck about a rotational axis, wherein the substrate comprises a substantially planar surface comprising the plurality of biological analytes immobilized thereto, wherein a fluid outlet and an objective are not in contact with the substrate and are respectively disposed at a first location and a second location above the substantially planar surface of the substrate;
(b) dispensing a solution to the substantially planar surface of the substrate, using the fluid outlet at the first location, to contact at least a subset of the plurality of biological analytes, wherein the solution disperses radially outwards from a location of dispense, corresponding to the first location of the fluid outlet, on the substantially planar surface relative to the rotational axis during or subsequent to rotation of the substrate; and
(c) imaging the substantially planar surface of the substrate using the objective at the second location to detect one or more signals or signal changes, or lack thereof, from the substrate to process a biological analyte of the plurality of biological analytes.