US 12,480,859 B2
Methods and systems for evaluating flow cytometer data for the presence of a coincident event
Pierce O. Norton, Los Gatos, CA (US)
Assigned to BECTON, DICKINSON AND COMPANY, Franklin Lakes, NJ (US)
Filed by Becton, Dickinson and Company, Franklin Lakes, NJ (US)
Filed on Jan. 10, 2023, as Appl. No. 18/095,130.
Claims priority of provisional application 63/313,391, filed on Feb. 24, 2022.
Prior Publication US 2023/0266228 A1, Aug. 24, 2023
Int. Cl. G01N 15/1434 (2024.01); G01N 15/01 (2024.01); G01N 15/10 (2024.01)
CPC G01N 15/1436 (2013.01) [G01N 15/01 (2024.01); G01N 2015/1006 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method of evaluating flow cytometer data for the presence of a coincident event, the method comprising:
irradiating first and second particles in a flow stream with first and second light sources, respectively;
receiving:
flow cytometer data associated with the first particle, wherein the flow cytometer data is obtained from a detector following the irradiation of the first particle and the second particle in the flow stream by the first and second light sources, respectively; and
first and second trigger signals associated with the first and second particles, respectively;
generating a time interval for the first and second particles based on a difference between the first and second trigger signals; and
evaluating the flow cytometer data for the presence of a coincident event based on the generated time interval by determining if the first and second particles are irradiated by the first and second light sources simultaneously.