| CPC C12Q 1/701 (2013.01) [C12Q 1/686 (2013.01); C12Q 1/6876 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01)] | 8 Claims |
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1. A kit for multiplex detection of novel coronavirus 2019-nCOV nucleic acid, which comprises a primer pair set and a probe set,
wherein the primer pair set comprises:
a first primer pair group comprising:
a forward primer as shown in SEQ ID NO: 1; and a reverse primer as shown in SEQ ID NO: 2;
a second primer pair group comprising:
a forward primer as shown in SEQ ID NO: 4 and a reverse primer as shown in SEQ ID NO: 5;
a third primer pair group comprising:
a forward primer as shown in SEQ ID NO: 7 and a reverse primer as shown in SEQ ID NO: 8; and
an internal standard primer pair group comprising:
a forward primer as shown in SEQ ID NO: 10 and a reverse primer as shown in SEQ ID NO: 11; and
the probe set comprises:
a first probe whose nucleotide sequence is shown in SEQ ID NO: 3, the 5′ end of the first probe is labeled with a first fluorescent reporter group, and the 3′ end of the first probe is labeled with a first fluorescence quenching group;
a second probe whose nucleotide sequence is shown in SEQ ID NO: 6, the 5′ end of the second probe is labeled with a second fluorescent reporter group, and the 3′ end of the second probe is labeled with a second fluorescence quenching group;
a third probe whose nucleotide sequence is shown in SEQ ID NO: 9, the 5′ end of the third probe is labeled with a third fluorescent reporter group, and the 3′ end of the third probe is labeled with a third fluorescence quenching group; and
an internal control probe whose nucleotide sequence is shown in SEQ ID NO: 12, the 5′ end of the internal control probe is labeled with a fourth fluorescent reporter group, and the 3′ end of the internal control probe is labeled with a fourth fluorescence quenching group,
wherein fluorescence channels of the first to the fourth fluorescent reporter groups are different from each other.
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