US 12,480,164 B2
DNA methylation biomarkers for early detection of cervical cancer
Moshe Szyf, Cote St Luc (CA); David Cheishvili, Pierrefonds (CA); Eduardo Franco, Montreal (CA); and Mariam El-Zein, Montreal (CA)
Assigned to EpiMedTechGlobal (EMTG), Singapore (SG)
Appl. No. 17/299,132
Filed by HKG EPITHERAPEUTICS LIMITED, Pierrefonds (CA)
PCT Filed Feb. 4, 2020, PCT No. PCT/IB2020/050885
§ 371(c)(1), (2) Date Jun. 2, 2021,
PCT Pub. No. WO2020/115728, PCT Pub. Date Jun. 11, 2020.
Claims priority of provisional application 62/774,994, filed on Dec. 4, 2018.
Prior Publication US 2022/0073994 A1, Mar. 10, 2022
Int. Cl. C12Q 1/6886 (2018.01); G16B 20/20 (2019.01)
CPC C12Q 1/6886 (2013.01) [G16B 20/20 (2019.02); C12Q 2600/154 (2013.01)] 5 Claims
 
1. A kit for predicting cervical cancer, comprising reagents and primers for DNA methylation measurements of CGIDs as set forth in SEQ ID NO:3, SEQ ID NO:31, and a combination thereof, wherein the reagents and primers are selected from:
a) a forward, biotinylated primer consisting of the nucleotide sequence SEQ ID NO:80, a reverse primer consisting of the nucleotide sequence SEQ ID NO:81, and a pyrosequencing primer consisting of the nucleotide sequence SEQ ID NO:82, for DNA methylation measurements of the CGIDs as set forth in SEQ ID NO: 31;
b) a forward primer consisting of the nucleotide sequence SEQ ID NO:90, and a reverse primer consisting of the nucleotide sequence SEQ ID NO:91, for DNA methylation measurements of the CGIDs as set forth in SEQ ID NO: 31;
c) a forward primer consisting of the nucleotide sequence SEQ ID NO:83, a reverse primer consisting of the nucleotide sequence SEQ ID NO:84, and a pyrosequencing primer consisting of the nucleotide sequence SEQ ID NO:85, for DNA methylation measurements of the CGIDs as set forth in SEQ ID NO: 3;
d) a forward primer consisting of the nucleotide sequence SEQ ID NO:88 and a reverse primer consisting of the nucleotide sequence SEQ ID NO:89, for DNA methylation measurements of the CGIDs as set forth in SEQ ID NO: 3; or
a combination of any one of a)-d).