US 12,480,159 B2
Compositions containing identifier sequences on solid supports for nucleic acid sequence analysis
Charles R. Cantor, Del Mar, CA (US)
Assigned to Sequenom, Inc., San Diego, CA (US)
Filed by Sequenom, Inc., San Diego, CA (US)
Filed on Jun. 1, 2023, as Appl. No. 18/327,087.
Application 18/327,087 is a continuation of application No. 16/912,041, filed on Jun. 25, 2020, granted, now 11,708,607.
Application 16/912,041 is a continuation of application No. 14/973,467, filed on Dec. 17, 2015, granted, now 10,731,213, issued on Aug. 4, 2020.
Application 14/973,467 is a continuation of application No. 14/687,790, filed on Apr. 15, 2015, granted, now 10,144,966, issued on Dec. 4, 2018.
Application 14/687,790 is a continuation of application No. 12/354,749, filed on Jan. 15, 2009, granted, now 9,034,580, issued on May 19, 2015.
Claims priority of provisional application 61/021,871, filed on Jan. 17, 2008.
Prior Publication US 2024/0150833 A1, May 9, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6876 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6844 (2018.01)
CPC C12Q 1/6876 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6844 (2013.01)] 28 Claims
 
1. A method for analyzing a sample nucleic acid, wherein the method comprises:
(a) contacting a biological sample comprising the sample nucleic acid with a collection of solid supports,
wherein each solid support in the collection comprises at least 10 different single-stranded nucleic acid species linked to the solid support, wherein each single stranded nucleic acid species linked to a common solid support comprises an identifier sequence and a probe sequence,
wherein all the single-stranded nucleic acid species linked to a common solid support share the same identifier sequence,
wherein the identifier sequence from the at least 10 different single-stranded nucleic acid species linked to the common solid support in the collection is different from the identifier sequences of the at least 10 different single-stranded nucleic acid species linked to other solid supports in the collection, whereby each solid support in the collection is associated with a unique identifier sequence,
wherein the probe sequence of each of the at least 10 different single-stranded nucleic acid species linked to the solid support allows each of the at least 10 different single-stranded nucleic acid species to hybridize to a subsequence in a sample nucleic acid,
thereby producing a mixture in which each of the at least 10 different single-stranded nucleic acid species on the solid support in the collection hybridizes to a subsequence of the sample nucleic acid sequence when the probe sequence is complementary to a nucleotide subsequence in the sample nucleic acid sequence; and
(b) contacting the mixture with extension reagents under conditions in which solid phase nucleic acid species hybridized to molecules of the sample nucleic acid are extended by the reagents, thereby producing extended solid phase nucleic acid species comprising the unique identifier sequence of the solid support and a complementary sequence of the sample nucleic acid.