US 12,480,147 B2
Biosynthetic production of gamma-lactones
Hui Chen, North Billerica, MA (US); and Oliver Yu, Lexington, MA (US)
Assigned to Conagen Inc., Bedford, MA (US)
Filed by Conagen Inc., Bedford, MA (US)
Filed on Dec. 7, 2022, as Appl. No. 18/062,628.
Application 18/062,628 is a division of application No. 17/150,774, filed on Jan. 15, 2021, granted, now 11,549,131.
Application 17/150,774 is a continuation of application No. PCT/US2019/042300, filed on Jul. 17, 2019.
Claims priority of provisional application 62/758,019, filed on Nov. 9, 2018.
Claims priority of provisional application 62/699,374, filed on Jul. 17, 2018.
Prior Publication US 2024/0076702 A1, Mar. 7, 2024
Int. Cl. C12P 17/04 (2006.01); C12N 15/52 (2006.01)
CPC C12P 17/04 (2013.01) [C12N 15/52 (2013.01)] 9 Claims
 
1. A method for producing a C4-C20 gamma lactone, the method comprising:
providing a reaction mixture comprising a recombinant CYP450 protein having carboxylic acid 4-hydroxylase activity and a C4-C20 carboxylic acid substrate to provide a 4-hydroxy C4-C20 carboxylic acid; and
subjecting the 4-hydroxy C4-C20 carboxylic acid to acidic conditions to produce the C4-C20 gamma lactone;
wherein the recombinant CYP450 protein comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 3 or SEQ ID NO: 5.