US 12,480,131 B2
Tropane alkaloid (TA) producing non-plant host cells, and methods of making and using the same
Christina D. Smolke, Menlo Park, CA (US); and Prashanth Srinivasan, Stanford, CA (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Appl. No. 17/436,546
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
PCT Filed Mar. 6, 2020, PCT No. PCT/US2020/021577
§ 371(c)(1), (2) Date Sep. 3, 2021,
PCT Pub. No. WO2020/185626, PCT Pub. Date Sep. 17, 2020.
Claims priority of provisional application 62/891,771, filed on Aug. 26, 2019.
Claims priority of provisional application 62/848,419, filed on May 15, 2019.
Claims priority of provisional application 62/815,709, filed on Mar. 8, 2019.
Prior Publication US 2022/0170026 A1, Jun. 2, 2022
Int. Cl. C12N 15/52 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 9/06 (2006.01); C12N 9/10 (2006.01); C12N 9/78 (2006.01); C12N 9/88 (2006.01); C12P 17/12 (2006.01); C12P 17/18 (2006.01)
CPC C12N 15/52 (2013.01) [C12N 9/0006 (2013.01); C12N 9/0022 (2013.01); C12N 9/0032 (2013.01); C12N 9/0071 (2013.01); C12N 9/1007 (2013.01); C12N 9/78 (2013.01); C12N 9/88 (2013.01); C12P 17/12 (2013.01); C12P 17/188 (2013.01); C12Y 101/01027 (2013.01); C12Y 101/01206 (2013.01); C12Y 101/01236 (2013.01); C12Y 101/01237 (2013.01); C12Y 102/01003 (2013.01); C12Y 104/0301 (2013.01); C12Y 104/03021 (2013.01); C12Y 104/03022 (2013.01); C12Y 105/03017 (2013.01); C12Y 106/02004 (2013.01); C12Y 114/11011 (2013.01); C12Y 201/01053 (2013.01); C12Y 203/01001 (2013.01); C12Y 203/01035 (2013.01); C12Y 204/02028 (2013.01); C12Y 205/01022 (2013.01); C12Y 206/01057 (2013.01); C12Y 207/07009 (2013.01); C12Y 207/11001 (2013.01); C12Y 305/01053 (2013.01); C12Y 305/03001 (2013.01); C12Y 305/03011 (2013.01); C12Y 305/03012 (2013.01); C12Y 401/01001 (2013.01); C12Y 401/01017 (2013.01); C12Y 401/01019 (2013.01); C12Y 402/01051 (2013.01); C12Y 403/01024 (2013.01); C12Y 403/01025 (2013.01)] 19 Claims
 
1. An engineered non-plant cell that produces a precursor of a tropane alkaloid product, a tropane alkaloid product, or a derivative of a tropane alkaloid product, wherein the engineered non-plant cell comprises a heterologous coding sequences encoding hyoscyamine dehydrogenase (HDH) within a pathway for producing the precursor of a tropane alkaloid product, the tropane alkaloid product, or the derivative of a tropane alkaloid product.