| CPC C12N 15/113 (2013.01) [A61K 9/0048 (2013.01); A61K 31/7105 (2013.01); C12N 15/11 (2013.01); C12N 2310/11 (2013.01); C12N 2310/20 (2017.05); C12N 2310/315 (2013.01); C12N 2310/321 (2013.01); C12N 2310/3231 (2013.01); C12N 2310/3515 (2013.01)] | 10 Claims |
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1. A method of treating a subject with Fuchs endothelial corneal dystrophy comprising administering a gene editing construct and two guide RNAs that target intron 2 of TCF4 or the 3′-UTR of DMPK, wherein said gene editing construct excises the CTG tract repeat.
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