	US 12,480,112 B2
	Glutamate decarboxylase mutant with improved pH tolerance and use thereof in synthesis of gamma-aminobutyric acid
Zhiming Rao, Wuxi (CN); Jin Han, Wuxi (CN); Taowei Yang, Wuxi (CN); Meijuan Xu, Wuxi (CN); and Xian Zhang, Wuxi (CN)
Assigned to JIANGNAN UNIVERSITY, Jiangsu (CN)
Filed by JIANGNAN UNIVERSITY, Wuxi (CN)
Filed on Dec. 15, 2023, as Appl. No. 18/542,109.
Application 18/542,109 is a continuation of application No. PCT/CN2022/076360, filed on Feb. 15, 2022.
Claims priority of application No. 202210086048.3 (CN), filed on Jan. 25, 2022.
Prior Publication US 2024/0110173 A1, Apr. 4, 2024
Int. Cl. C12N 9/88 (2006.01); C12N 1/21 (2006.01); C12N 15/60 (2006.01); C12P 13/00 (2006.01)

CPC C12N 9/88 (2013.01) [C12P 13/005 (2013.01); C12Y 401/01015 (2013.01)]

10 Claims

1. A glutamate decarboxylase mutant with a broadened pH range, the decarboxylase mutant having one of the following mutations relative to the amino acid sequence as shown in SEQ ID NO: 3:

(1) serine at position 24 mutated to arginine;

(2) serine at position 24 mutated to arginine, and aspartic acid at position 88 mutated to arginine;

(3) serine at position 24 mutated to arginine, and tyrosine at position 309 mutated to lysine; and

(4) serine at position 24 mutated to arginine, aspartic acid at position 88 mutated to arginine, and tyrosine at position 309 mutated to lysine.