	US 12,480,101 B2
	Modified host cells for high efficiency production of vanillin
Lauren Raetz, Emeryville, CA (US); and Chad Hansen, Emeryville, CA (US)
Assigned to AMYRIS, INC., Emeryville, CA (US)
Appl. No. 17/631,429
Filed by AMYRIS, INC., Emeryville, CA (US)
PCT Filed Jul. 31, 2020, PCT No. PCT/US2020/044613 § 371(c)(1), (2) Date Jan. 28, 2022, PCT Pub. No. WO2021/022216, PCT Pub. Date Feb. 4, 2021.
Claims priority of provisional application 62/881,874, filed on Aug. 1, 2019.
Prior Publication US 2022/0282226 A1, Sep. 8, 2022
Int. Cl. C12N 9/02 (2006.01); C07C 47/58 (2006.01); C12P 7/24 (2006.01)

CPC C12N 9/0008 (2013.01) [C07C 47/58 (2013.01); C12P 7/24 (2013.01); C12Y 102/01003 (2013.01)]

15 Claims

1. A genetically modified Saccharomyces cerevisiae host cell that produces vanillin or glucovanillin, the host cell comprising:

(i) deletion of a homolog of fatty aldehyde dehydrogenase (HFD1) gene; and

(ii) one or more nucleic acids expressing:

a catechol-O-methyltransferase (COMT);

an aromatic carboxylic acid reductase (ACAR) from Nocardia iowensis;

a phosphopantetheinyl transferase (PPTASE); and

a Podospora pauciseta 3-dehydroshikimate dehydratase (AroZ),

wherein the host cell does not express an Hfd1 protein.