Ketoreductase polypeptides for the preparation of phenylephrine
Oscar Alvizo, Fremont, CA (US); Steven J. Collier, Concord, MA (US); Hans-Georg Joerg Hennemann, Bedburg (DE); Seong Ho Oh, Singapore (SG); and Wenjuan Zha, Singapore (SG)
Assigned to Codexis, Inc., Redwood City, CA (US)
Filed by Codexis, Inc., Redwood City, CA (US)
Filed on May 22, 2024, as Appl. No. 18/671,324.
Application 14/755,056 is a division of application No. 13/390,677, granted, now 9,102,959, issued on Aug. 11, 2015, previously published as PCT/US2010/046020, filed on Aug. 19, 2010.
Application 18/671,324 is a continuation of application No. 17/731,635, filed on Apr. 28, 2022, granted, now 12,031,159.
Application 17/731,635 is a continuation of application No. 17/085,783, filed on Oct. 30, 2020, granted, now 11,345,898, issued on May 11, 2022.
Application 17/085,783 is a continuation of application No. 16/778,368, filed on Jan. 31, 2020, granted, now 10,870,835, issued on Dec. 22, 2020.
Application 16/778,368 is a continuation of application No. 16/437,354, filed on Jun. 11, 2019, granted, now 10,590,396, issued on Mar. 17, 2020.
Application 16/437,354 is a continuation of application No. 15/812,628, filed on Nov. 14, 2017, granted, now 10,358,631, issued on Jul. 23, 2019.
Application 15/812,628 is a continuation of application No. 14/755,056, filed on Jun. 30, 2015, granted, now 9,834,758, issued on Dec. 5, 2017.
Claims priority of provisional application 61/235,324, filed on Aug. 19, 2009.
Prior Publication US 2024/0301366 A1, Sep. 12, 2024
1. An engineered polypeptide capable of converting 1-(3-hydroxyphenyl)-2-(methylamino) ethanone to (R)-phenylephrine, wherein the amino acid sequence of the polypeptide has at least 90% identity to SEQ ID NO: 2 and comprises a residue difference E145A relative to SEQ ID NO: 2.