	US 12,479,878 B2
	Crystallization of 2′-FL
Getachew S. Molla, Hørsholm (DK); and Pierre Chassagne, Hørsholm (DK)
Assigned to GLYCOM A/S, Hørsholm (DK)
Appl. No. 17/906,131
Filed by Glycom A/S, Hørsholm (DK)
PCT Filed Mar. 12, 2021, PCT No. PCT/IB2021/052073 § 371(c)(1), (2) Date Sep. 12, 2022, PCT Pub. No. WO2021/181349, PCT Pub. Date Sep. 16, 2021.
Claims priority of application No. PA 2020 00335 (DK), filed on Mar. 12, 2020.
Prior Publication US 2023/0114668 A1, Apr. 13, 2023
Int. Cl. C07H 3/00 (2006.01); C07H 1/06 (2006.01); C07H 3/06 (2006.01); C13B 30/02 (2011.01)

CPC C07H 3/06 (2013.01) [C07H 1/06 (2013.01); C13B 30/021 (2013.01)]

20 Claims

1. A method for crystallizing 2′-FL comprising:

i) providing an aqueous solution or syrup comprising 2′-FL at a temperature of 35-60° C.,

ii) while maintaining the temperature of the aqueous solution or syrup at 35-60° C., adding a 10-30 wt % suspension of crystalline 2′-FL in acetic acid to the aqueous solution or syrup comprising 2′-FL, thereby generating a slurry wherein the amount of acetic acid in the slurry, after addition of the suspension of crystalline 2′-FL in acetic acid is 1.5-4 wt %,

iii) at a temperature of 35-60° C., adding acetic acid to the slurry until the ratio of acetic acid to water is 4-6 to form a diluted slurry,

iv) obtaining a crystalline mass from the diluted slurry, and

v) filtering the crystalline 2′-FL from the crystalline mass.