	US 12,478,967 B2
	Method and apparatus for processing tissue and other samples encoding cellular spatial position information
Stevan Bogdan Jovanovich, Livermore, CA (US)
Assigned to SILICON VALLEY SCIENTIFIC, INC., Livermore, CA (US)
Filed by SILICON VALLEY SCIENTIFIC, INC., Livermore, CA (US)
Filed on Sep. 4, 2024, as Appl. No. 18/824,062.
Application 18/824,062 is a continuation of application No. 16/980,358, granted, now 12,103,004, previously published as PCT/US2019/021942, filed on Mar. 12, 2019.
Claims priority of provisional application 62/670,234, filed on May 11, 2018.
Claims priority of provisional application 62/641,425, filed on Mar. 12, 2018.
Prior Publication US 2025/0242350 A1, Jul. 31, 2025
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 3/00 (2006.01); C12N 15/10 (2006.01); G01N 1/28 (2006.01); G01N 35/00 (2006.01); G01N 35/08 (2006.01); G16B 5/00 (2019.01)

CPC B01L 3/502784 (2013.01) [B01L 3/502761 (2013.01); B01L 3/50857 (2013.01); C12N 15/1013 (2013.01); G01N 1/286 (2013.01); G01N 35/0099 (2013.01); G01N 35/08 (2013.01); G16B 5/00 (2019.02); B01L 2200/025 (2013.01); B01L 2200/026 (2013.01); B01L 2200/027 (2013.01); B01L 2200/028 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/0896 (2013.01); B01L 2300/18 (2013.01)]

20 Claims

1. A method comprising:

a) providing a biological tissue specimen on a sample carrier, wherein cells in the tissue sample have a spatial position in the tissue specimen, and wherein the sample carrier comprises (i) a perforated support that supports the tissue specimen and (ii) an array of passages at addressable positions through the sample carrier and positioned below the perforated support;

b) disrupting cells in the tissue specimen to release one or a plurality of cells or nuclei; and

c) collecting a microsample comprising one or a plurality of released cells or nuclei through the perforations into the passages, wherein the addressable position of a passage indicates the original spatial position of the cell or nuclei moved into the passage.