US 12,478,964 B2
Device for the analysis of nucleic acid molecules
Vincent Croquette, Antony (FR); David Bensimon, Paris (FR); Jimmy Ouellet, Lardy (FR); Jean-François Allemand, Bourg la Reine (FR); Charles Andre, Paris (FR); and Gordon Hamilton, Paris (FR)
Assigned to Centre National de la Recherche Scientifique (CNRS), (FR); Sorbonne Universite, (FR); Paris Sciences et Lettres, (FR); and UNIVERSITÉ PARIS CITÉ, (FR)
Filed by Paris Sciences Et Lettres—Quartier Latin, Paris (FR); Centre National de la Recherche Scientifique (CNRS), Paris (FR); Universite Paris Diderot Paris 7, Paris (FR); and Sorbonne Universite, Paris (FR)
Filed on Feb. 26, 2021, as Appl. No. 17/186,954.
Application 17/186,954 is a continuation of application No. 15/571,679, granted, now 10,933,416, previously published as PCT/EP2016/060155, filed on May 6, 2016.
Claims priority of application No. 15305710 (EP), filed on May 7, 2015.
Prior Publication US 2021/0178384 A1, Jun. 17, 2021
Int. Cl. B01L 3/00 (2006.01); C12Q 1/6825 (2018.01); G01N 27/07 (2006.01); G01N 27/327 (2006.01)
CPC B01L 3/50273 (2013.01) [C12Q 1/6825 (2013.01); G01N 27/3275 (2013.01); B01L 3/5085 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0829 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0436 (2013.01); C12Q 2523/307 (2013.01); C12Q 2525/301 (2013.01); C12Q 2563/116 (2013.01); C12Q 2563/155 (2013.01); G01N 27/07 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A device, comprising:
a plate having a top surface and a bottom surface, the plate comprising a well formed therein, the well extending along an axis into the plate from the top surface to a base of the well, the base being defined by the plate, the well opening at the top surface, the axis being normal to the bottom surface and the base of the well closing the well at the bottom surface;
a nucleic acid molecule disposed in the well, the nucleic acid molecule comprising a first end, the first end being anchored to a surface at or proximate to the base;
a bead disposed in the well, the nucleic acid molecule comprising a second end, the bead being anchored to the second end,
an actuator configured to cause the bead to move relative to the bottom surface along the axis within a portion of the well along the axis, the portion of the well having a cross-sectional area measured perpendicular to the axis, the cross-section area increasing as a distance from the base to the cross-section area increases;
and
a sensor configured to measure an impedance of the well, the impedance depending on a distance between the bead and the base.