US 12,478,669 B1
Herpes zoster mRNA vaccine, preparation method therefor, and use thereof
Gengshen Song, Zhejiang (CN); Kai Dong, Zhejiang (CN); Zhongbin Chen, Zhejiang (CN); Yanfen Li, Zhejiang (CN); Yuting Zhou, Zhejiang (CN); Jing Li, Zhejiang (CN); Xin Chai, Zhejiang (CN); Zhongcai Gao, Zhejiang (CN); Jinyu Zhang, Zhejiang (CN); and Huanyu Wang, Zhejiang (CN)
Assigned to Hangzhou Tianlong Pharmaceutical Co., Ltd., Hangzhou (CN)
Filed by Hangzhou Tianlong Pharmaceutical Co., Ltd., Zhejiang (CN)
Filed on Jan. 18, 2025, as Appl. No. 19/031,360.
Claims priority of application No. 202410659996.0 (CN), filed on May 27, 2024.
Int. Cl. A61K 39/25 (2006.01); A61K 9/51 (2006.01); A61K 31/7105 (2006.01); A61K 39/00 (2006.01); A61P 37/04 (2006.01); C12N 15/86 (2006.01)
CPC A61K 39/25 (2013.01) [A61P 37/04 (2018.01); A61K 2039/53 (2013.01); A61K 2039/54 (2013.01); A61K 2039/6018 (2013.01)] 49 Claims
 
1. An immunogenic composition, comprising a ribonucleic acid (RNA) of a varicella-zoster virus (VZV) encoding a variant of the wild-type VZV gE glycoprotein;
wherein the sequence of the wild-type VZV gE glycoprotein is SEQ ID NO: 3;
wherein the variant of the VZV gE glycoprotein is the variant selected from: YK-VZV-020, YK-VZV-030, YK-VZV-031, YK-VZV-009, YK-VZV-010, YK-VZV-011, YK-VZV-012, YK-VZV-013 and YK-VZV-014,
 
 
 Sequence
 
 Sequence
Name truncation Site mutation deletion
 
YK-VZV-020 gE (1 to 573) Y569K No sequence
 
 
 
 deletion
YK-VZV-030 gE (1 to 587) A568D; Y569K; R570E; No sequence
 
 
 V571K; Y582A deletion
YK-VZV-031 gE (1 to 587) A568D; Y569K; R570E; No sequence
 
 
 V571K; Y582G deletion
YK-VZV-009 No sequence Y569K; Y582A No sequence
 
 truncation
 
 deletion
YK-VZV-010 No sequence Y569K; Y582A; S593A; No sequence
 
 truncation S595A; T596A; T598A deletion
YK-VZV-011 No sequence A568D; Y569K; R570E; No sequence
 
 truncation V571K deletion
YK-VZV-012 No sequence A568D; Y569K; R570E; No sequence
 
 truncation V571K; S593A; S595A; deletion
 
 
 T596A; T598A
 
YK-VZV-013 No sequence A568D; Y569K; R570E; No sequence
 
 truncation V571K; Y582A deletion
YK-VZV-014 No sequence A568D; Y569K; R570E; No sequence
 
 truncation V571K; Y582A; S593A; deletion
 
 
 S595A; T596A; T598A.