US 12,478,655 B2
Olive derived cell culture and methods for preparing and using the same
Yoheved Hagay, Rehovot (IL); and Malkit Azachi, Rehovot (IL)
Assigned to BIO HARVEST LTD., Rehovot (IL)
Filed by BIO HARVEST LTD., Rehovot (IL)
Filed on Apr. 7, 2024, as Appl. No. 18/628,751.
Application 18/628,751 is a continuation of application No. 17/155,059, filed on Jan. 21, 2021, abandoned.
Application 17/155,059 is a continuation of application No. 16/072,886, abandoned, previously published as PCT/IL2017/050098, filed on Jan. 26, 2017.
Claims priority of provisional application 63/463,378, filed on May 2, 2023.
Claims priority of provisional application 62/287,453, filed on Jan. 27, 2016.
Prior Publication US 2024/0269216 A1, Aug. 15, 2024
Int. Cl. A61K 36/63 (2006.01); A01H 4/00 (2006.01); A01H 5/08 (2018.01); A01H 5/10 (2018.01); A61K 9/14 (2006.01); A61P 3/00 (2006.01); B65D 88/00 (2006.01); C11B 1/00 (2006.01); C12M 1/00 (2006.01); C12N 5/00 (2006.01)
CPC A61K 36/63 (2013.01) [A01H 4/00 (2013.01); A01H 5/08 (2013.01); A01H 5/10 (2013.01); A61K 9/14 (2013.01); A61P 3/00 (2018.01); C11B 1/00 (2013.01); C12N 5/0025 (2013.01); B65D 88/00 (2013.01); C12M 21/00 (2013.01); C12M 23/14 (2013.01)] 16 Claims
 
1. A process for the in vitro production of an olive cell culture of olive leaf, seed or olive fruit cells grown in vitro comprising:
growing olive cells in a flask;
inoculating the olive cells from the flask into a first bioreactor;
inoculating the olive cells from the first bioreactor into a second bioreactor;
optionally inoculating the olive cells from the second bioreactor into a last bioreactor; and
harvesting the olive cells from the last bioreactor;
wherein the second bioreactor is a last bioreactor or an intermediate bioreactor and the size of each bioreactor used in the process is larger than the one in which the olive cells were previously grown; wherein at least one bioreactor is a disposable bioreactor and wherein the disposable bioreactor includes an inner and an outer layer prepared from polyethylene and a middle layer prepared from nylon; wherein the olive cells harvested from the last bioreactor are dried and wherein the olive cells are grown in a modified MS growth medium enriched with casein hydrolysate and one or more of myo inositol, 1-naphthaleneacetic acid (NAA), kinetin, 2,4,D, NAA, BA, 2iP wherein the olive cell culture grown in vitro comprises verbascoside in the amount of at least 4.1 mg/gr dry weight.