US 12,478,405 B2
Centrifuge-free sperm preparation in an intelligent automated in vitro fertilization and intracytoplasmic sperm injection platform
Jacques Cohen, New York, NY (US); Alejandro Chavez-Badiola, Mexico City (MX); Gerardo Mendizabal-Ruiz, Guadalajara (MX); Cesar Millan, Zapopan (MX); Roberto Valencia-Murillo, Las Paz (MX); Vladimir C. Ocegueda Hernandez, Zapopan (MX); Nuno Costa-Borges, Barcelona (ES); Victor Manuel Medina Perez, Guadalajara (MX); Victor Manuel Rico Botero, Guadalajara (MX); and William Nicholas Garbarini, Jr., Cranford, NJ (US)
Assigned to Conceivable Life Sciences Inc., New York, NY (US)
Filed by Conceivable Life Sciences Inc., New York, NY (US)
Filed on Feb. 2, 2024, as Appl. No. 18/431,061.
Application 18/431,061 is a continuation of application No. PCT/US2024/013428, filed on Jan. 30, 2024.
Claims priority of provisional application 63/523,258, filed on Jun. 26, 2023.
Prior Publication US 2024/0425783 A1, Dec. 26, 2024
Int. Cl. A61B 17/43 (2006.01); A61B 17/425 (2006.01); A61B 34/30 (2016.01); C12M 1/00 (2006.01); C12M 1/02 (2006.01); C12M 1/22 (2006.01); C12M 1/26 (2006.01); C12M 1/36 (2006.01); C12M 3/00 (2006.01); C12N 5/073 (2010.01); G01N 1/10 (2006.01); G01N 33/50 (2006.01); G01N 35/00 (2006.01); G01N 35/10 (2006.01); G06T 7/20 (2017.01); G06T 7/70 (2017.01); G06V 10/82 (2022.01); G06V 20/69 (2022.01); A61B 34/32 (2016.01)
CPC A61B 17/43 (2013.01) [A61B 17/425 (2013.01); A61B 34/30 (2016.02); C12M 21/06 (2013.01); C12M 23/10 (2013.01); C12M 23/48 (2013.01); C12M 23/50 (2013.01); C12M 33/04 (2013.01); C12M 41/06 (2013.01); C12M 41/18 (2013.01); C12M 41/48 (2013.01); C12N 5/0604 (2013.01); G01N 33/5091 (2013.01); G01N 35/0099 (2013.01); G01N 35/10 (2013.01); G01N 35/1011 (2013.01); G06T 7/20 (2013.01); G06T 7/70 (2017.01); G06V 10/82 (2022.01); G06V 20/693 (2022.01); A61B 34/32 (2016.02); G06T 2207/20081 (2013.01); G06T 2207/30024 (2013.01); G06V 20/69 (2022.01); G06V 20/698 (2022.01); G06V 2201/03 (2022.01)] 23 Claims
 
1. A method of artificial-intelligence-based robotic pipetting for spermatozoa preparation and ranking, the method comprising:
positioning a vessel containing a semen sample within a staging mechanism;
using a robotic pipettor to dispense at least two droplets within a dish, wherein a selected droplet of the at least two droplets receives a portion of the semen sample;
using the robotic pipettor to fluidly connect the at least two droplets by moving a tip of the robotic pipettor between the at least two droplets to create a medium channel according to a programmable design commanded by an artificial intelligence/machine learning system (AI/ML system);
using the AI/ML system to optically scan a plurality of sperm from the portion of the semen sample to produce a first image object and a second image object using an imaging system, wherein the imaging system includes at least one of a microscopy system, a camera system, and a lighting system, and wherein creation of the first image object is separated by a specified time duration from creation of the second image object;
using the AI/ML system to compare at least one sperm component among the plurality of sperm in the first image object and the at least one sperm component among the plurality of sperm in the second image object to compute a real-time motility metric for the plurality of sperm based at least in part on a change in sperm location between the first image object and the second image object; and
ranking the plurality of sperm based at least in part on the real-time motility metric.