Ryan Philippe, Waltham, MA (US); Ajikumar Parayil Kumaran, Waltham, MA (US); Jason Donald, Waltham, MA (US); Krishna Patel, Waltham, MA (US); Swati Gupta, Waltham, MA (US); Ryan Lim, Waltham, MA (US); and Liwei Li, Waltham, MA (US)
Assigned to Manus Bio Inc., Waltham, MA (US)
Filed by Manus Bio Inc., Waltham, MA (US)
Filed on Sep. 14, 2023, as Appl. No. 18/467,362.
Application 16/251,993 is a division of application No. 15/524,015, granted, now 10,463,062, issued on Nov. 5, 2019, previously published as PCT/US2015/059273, filed on Nov. 5, 2015.
Application 18/467,362 is a continuation of application No. 16/862,214, filed on Apr. 29, 2020, granted, now 11,819,042.
Application 16/862,214 is a continuation of application No. 16/251,993, filed on Jan. 18, 2019, granted, now 10,743,567, issued on Aug. 18, 2020.
Claims priority of provisional application 62/075,644, filed on Nov. 5, 2014.
Prior Publication US 2024/0215615 A1, Jul. 4, 2024
1. A method for making rebaudioside M comprising: culturing a host cell in the presence of rebaudioside D, the host cell expressing a uridine diphosphate-dependent glycosyltransferase (UGT) enzyme comprising an amino acid sequence that has at least 90% sequence identity to SEQ ID NO: 3 and has a L200A or L200G substitution with respect to SEQ ID NO: 3.