	US 12,146,879 B2
	Capacitive biosensor for identifying a microorganism or determining antibiotic susceptibility
Kyung Hwa Yoo, Seoul (KR); Nal Ae Han, Busan (KR); Bong Jun Kim, Gyeonggi-do (KR); Sun Mi Lee, Seoul (KR); Kook Jin Lim, Seoul (KR); and Jeseung Oh, Gyeonggi-do (KR)
Assigned to INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY, Seoul (KR)
Filed by INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY, Seoul (KR); and PROTEOMETECH INC., Seoul (KR)
Filed on Oct. 12, 2020, as Appl. No. 17/068,148.
Application 17/068,148 is a division of application No. 15/675,953, filed on Aug. 14, 2017, abandoned.
Claims priority of application No. 10-2016-0103015 (KR), filed on Aug. 12, 2016.
Prior Publication US 2021/0140963 A1, May 13, 2021
Int. Cl. G01N 33/569 (2006.01); C12Q 1/04 (2006.01); C12Q 1/18 (2006.01); G01N 27/22 (2006.01); G01N 33/15 (2006.01); G01N 33/543 (2006.01); C12Q 1/00 (2006.01); G01N 33/00 (2006.01)

CPC G01N 33/569 (2013.01) [C12Q 1/04 (2013.01); C12Q 1/18 (2013.01); G01N 27/227 (2013.01); G01N 33/15 (2013.01); G01N 33/54366 (2013.01); G01N 33/5438 (2013.01); G01N 33/56911 (2013.01); C12Q 1/00 (2013.01); G01N 27/226 (2013.01); G01N 33/00 (2013.01); G01N 2500/10 (2013.01)]

18 Claims

1. A method for determining an antibiotic susceptibility of a microorganism, the method comprising:

binding the microorganism to a capacitive biosensor, the capacitive biosensor comprising:

a substrate including an anodic aluminum oxide;

an electrode layer formed on the substrate and including an interdigitated first electrode and an interdigitated second electrode; and

an aptamer fixed to the substrate;

treating the microorganism-bound capacitive biosensor with an antibiotic; and

determining a change in capacitance after the antibiotic treatment,

wherein the aptamer is fixed between the first electrode and the second electrode.