US 12,146,876 B2
Solid supports for decoding methods for multiplexing assays
John Michael Ramsey, Chapel Hill, NC (US); William Hampton Henley, Chapel Hill, NC (US); and Thomas Linz, Chapel Hill, NC (US)
Assigned to The University of North Carolina at Chapel Hill, Chapel Hill, NC (US)
Filed by The University of North Carolina at Chapel Hill, Chapel Hill, NC (US)
Filed on Apr. 29, 2021, as Appl. No. 17/244,257.
Application 17/244,257 is a division of application No. 15/765,749, granted, now 11,016,084, previously published as PCT/US2016/055407, filed on Oct. 5, 2016.
Claims priority of provisional application 62/237,324, filed on Oct. 5, 2015.
Prior Publication US 2022/0011300 A1, Jan. 13, 2022
Int. Cl. C12Q 1/68 (2018.01); G01N 33/542 (2006.01); G01N 33/543 (2006.01)
CPC G01N 33/542 (2013.01) [G01N 33/54313 (2013.01)] 17 Claims
 
1. A plurality of solid supports comprising:
a first population of solid supports comprising at least one encoding agent at a first concentration; and
a second population of solid supports comprising the at least one encoding agent at a second concentration and at least one additional encoding agent,
wherein the second population of solid supports comprises the at least one encoding agent at a ratio to the at least one additional encoding agent, and
wherein the fluorescence excitation and emission wavelengths for the at least one encoding agent and the at least one additional encoding agent respectively overlap, and
wherein the at least one encoding agent has a different stability in response to a defined event that causes a physical and/or chemical change in an encoding signal associated with the at least one encoding agent compared to the at least one additional encoding agent such that the population of solid supports encoded with the at least one encoding agent is distinguishable from the population of solid supports encoded with the at least one additional encoding agent after the defined event, and further wherein the defined event is a thermal, optical, or chemical event.