US 12,146,874 B2
Microtentacle imaging in patient tumor samples
Stuart S. Martin, Severna Park, MD (US); Christopher Jewell, Silver Spring, MD (US); James Andorko, Delran, NJ (US); Elisabeth Sooklal, Reisterstown, MD (US); Rebecca Whipple Bettes, Elkridge, MD (US); and Kristi Chakrabarti, Silver Spring, MD (US)
Assigned to UNIVERSITY OF MARYLAND, BALTIMORE, Baltimore, MD (US); and UNIVERSITY OF MARYLAND, COLLEGE PARK, College Park, MD (US)
Appl. No. 15/301,587
Filed by University of Maryland, Baltimore, Baltimore, MD (US)
PCT Filed Apr. 3, 2015, PCT No. PCT/US2015/024207
§ 371(c)(1), (2) Date Oct. 3, 2016,
PCT Pub. No. WO2015/153948, PCT Pub. Date Oct. 8, 2015.
Claims priority of provisional application 61/974,753, filed on Apr. 3, 2014.
Claims priority of provisional application 62/068,034, filed on Oct. 24, 2014.
Prior Publication US 2017/0184568 A1, Jun. 29, 2017
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/50 (2006.01); G01N 21/95 (2006.01); G01N 33/574 (2006.01); G02B 21/00 (2006.01)
CPC G01N 33/5026 (2013.01) [G01N 21/95 (2013.01); G01N 33/50 (2013.01); G01N 33/574 (2013.01); G02B 21/0028 (2013.01); G01N 2800/7028 (2013.01)] 36 Claims
 
1. A method for imaging microtentacles on cells taken directly from a primary solid tumor in a cancer patient to provide prognostic information and guide the patient's treatment comprising:
i) obtaining one or more live primary tumor cells that has been isolated by dissociation ex vivo from the primary solid tumor in the patient; and
ii) imaging the one or more live primary tumor cells and detecting the microtentacles.