US 12,146,869 B2
Methods and compositions for analyte quantification
Timothy Kassis, Saugus, MA (US); Jefferson Pruyne, Somerville, MA (US); Mark D. Simon, Somerville, MA (US); Mimoun Cadosch Delmar Akerman, Brookline, MA (US); Jennifer Campbell, Arlington, MA (US); Ana Henriques Da Costa, Cambridge, MA (US); Laura Kolinsky, Boston, MA (US); and John M. Geremia, Wayland, MA (US)
Assigned to MATTERWORKS INC, Somerville, MA (US)
Filed by MATTERWORKS INC, Somerville, MA (US)
Filed on Jul. 14, 2023, as Appl. No. 18/352,610.
Application 18/352,610 is a continuation of application No. 17/930,338, filed on Sep. 7, 2022, granted, now 11,754,536.
Claims priority of provisional application 63/371,978, filed on Aug. 19, 2022.
Claims priority of provisional application 63/274,513, filed on Nov. 1, 2021.
Prior Publication US 2024/0167986 A1, May 23, 2024
Int. Cl. G01N 30/72 (2006.01); C12M 1/34 (2006.01); G01N 30/86 (2006.01); G01N 30/02 (2006.01); G01N 30/04 (2006.01)
CPC G01N 30/72 (2013.01) [C12M 41/30 (2013.01); G01N 30/7233 (2013.01); G01N 30/8665 (2013.01); G01N 2030/027 (2013.01); G01N 2030/045 (2013.01)] 1 Claim
OG exemplary drawing
 
1. A kit for metabolomic analysis of a sample, comprising:
one or more calibrators configured to be used with a mass spectrometry (MS) machine equipped with a molecular ion source to quantify a plurality of metabolites associated with one or more metabolic pathways, wherein a number of the plurality of metabolites is greater than a number of the one or more calibrators, and the one or more calibrators comprise at least six calibrators selected from the group consisting of 1,4-Butanediamine (putrescine)·2HCl (13C4, 99%), LAlanine (13C3, 99%; 15N, 99%), Ethanolamine·HCl (1,1,2,2-D4, 98%), Sodium pyruvate (13C3, 99%), Creatinine (N-methyl-D3, 98%), Fumaric acid (13C4, 99%), Vitamin B3 (nicotinamide) (13C6, 99%), Thymine (1,3-15N2, 98%), L-Leucine (13C6, 99%), Hypoxanthine (13C5, 99%), LPhenylalanine (ring-13C6, 99%), Indole-3-acetic acid (phenyl-13C6, 99%), L-Tyrosine (ring-13C6, 99%), a-Ketoglutaric acid, disodium salt (1,2,3,4-13C4, 99%) CP 97%, Citric acid (1,5,6-carboxyl-13C3, 99%), L-Tryptophan (13Cl 1, 99%), Guanosine·2H20 (15N5, 96-98%), Sodium palmitate (U-13C 16, 98%), 6-diazo-5-oxo-L-norleucine, Acarbose, Aucubin, caffeine, Carbazochrome sodium sulfonate, Cefoselis Sulfate, Cellobiose, Ciprofloxacin hydrochloride hydrate, Cromolyn sodium, Cyclo (−RGDfK), Daminozide, Diminazene Aceturate, Fudosteine, Geniposidic acid, Gimeracil, Isoprinosine, Oseltamivir acid, Pidotimod, Puerarin, Sodium 4-amiropparaty Hyalrate, Telaglenastat, Tipiracil hydrochloride, Voglibose, and Zanamivir.